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Glial Growth Factor 2 induces proliferation and structural changes in ensheathing cells


Chuah, MI and Cossins, J-M and Woodhall, E and Tennent, R and Nash, G and West, AK, Glial Growth Factor 2 induces proliferation and structural changes in ensheathing cells, Brain Research, 857, (1-2) pp. 265-274. ISSN 0006-8993 (2000) [Refereed Article]

DOI: doi:10.1016/S0006-8993(99)02455-5


Ensheathing cells were isolated from neonatal rat olfactory bulbs and cultured in the presence of glial growth factor 2 (GGF2). Proliferation assay showed that at concentrations of up to 60 ng/ml GGF2, ensheathing cells underwent a modest increase in proliferation rate. This stimulation was not maintained at high doses of GGF2 at 100 ng/ml or more. Chemotaxis chambers and scanning electron microscopy were used to determine whether GGF2 was a chemoattractant for ensheathing cells. Although the results showed no chemotactic response to GGF2, ensheathing cells demonstrated structural changes when cultured in the presence of 20 ng/ml GGF2. Ultrastructural observations revealed that GGF2 promoted increased deposition of extracellular matrix on the cell membrane, more cytoskeletal elements in the processes and as a possible consequence, contributed to a more rigid support. Ensheathing cells cultured in the absence of GGF2 often extended thinner and curved processes. Reverse transcription-polymerase chain reaction confirmed the presence of GGF2 transcripts in ensheathing cells, suggesting that ensheathing cells themselves are a source of GGF2. Copyright (C) 2000 Elsevier Science B.V.

Item Details

Item Type:Refereed Article
Research Division:Biomedical and Clinical Sciences
Research Group:Neurosciences
Research Field:Cellular nervous system
Objective Division:Health
Objective Group:Clinical health
Objective Field:Clinical health not elsewhere classified
UTAS Author:Chuah, MI (Associate Professor Inn Chuah)
UTAS Author:Cossins, J-M (Dr Jo-Maree Courtney)
UTAS Author:Woodhall, E (Miss Emma Woodhall)
UTAS Author:Tennent, R (Mr Robert Tennent)
UTAS Author:West, AK (Professor Adrian West)
ID Code:19714
Year Published:2000
Web of Science® Times Cited:30
Deposited By:Biochemistry
Deposited On:2000-08-01
Last Modified:2013-12-04

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