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Glial Growth Factor 2 induces proliferation and structural changes in ensheathing cells
journal contribution
posted on 2023-05-16, 12:20 authored by Meng Inn ChuahMeng Inn Chuah, Jo-Maree CourtneyJo-Maree Courtney, Woodhall, E, Tennent, R, Nash, G, Adrian WestAdrian WestEnsheathing cells were isolated from neonatal rat olfactory bulbs and cultured in the presence of glial growth factor 2 (GGF2). Proliferation assay showed that at concentrations of up to 60 ng/ml GGF2, ensheathing cells underwent a modest increase in proliferation rate. This stimulation was not maintained at high doses of GGF2 at 100 ng/ml or more. Chemotaxis chambers and scanning electron microscopy were used to determine whether GGF2 was a chemoattractant for ensheathing cells. Although the results showed no chemotactic response to GGF2, ensheathing cells demonstrated structural changes when cultured in the presence of 20 ng/ml GGF2. Ultrastructural observations revealed that GGF2 promoted increased deposition of extracellular matrix on the cell membrane, more cytoskeletal elements in the processes and as a possible consequence, contributed to a more rigid support. Ensheathing cells cultured in the absence of GGF2 often extended thinner and curved processes. Reverse transcription-polymerase chain reaction confirmed the presence of GGF2 transcripts in ensheathing cells, suggesting that ensheathing cells themselves are a source of GGF2. Copyright (C) 2000 Elsevier Science B.V.
History
Publication title
Brain ResearchVolume
857Issue
1-2Pagination
265-274ISSN
0006-8993Department/School
Tasmanian School of MedicinePublisher
Elsevier SciencePlace of publication
NetherlandsRepository Status
- Restricted