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Sperm survival during short-term storage and after cryopreservation of semen from striped trumpeter ( Latris lineata)
journal contribution
posted on 2023-05-16, 12:16 authored by Ritar, AJ, Campet, MMethods of short-term storage and cryopreservation were examined for semen from striped trumpeter (Latris lineata). For fresh semen at 18°C, the percentage of motile sperm declined rapidly from over 80% immediately after activation with sea water to less than 2% within 9 min after activation. The motility after activation of undiluted fresh sperm stored at 5°C was maintained for two days and then declined markedly so that by the eighth day, sperm were mostly immotile after activation. The post-thawing motility was higher for sperm frozen with a non-activating diluent containing 2.84 M DMSO in saline (117 mM NaCl) than in an activating glycerol (2 M) medium in dilute sea water (300 mOsm). Post-thawing motility was higher for a dilution rate of 1:5 (semen:diluent) than 1:2 or 1:11 but was similar when frozen semen was thawed at 10°, 20°or 30°C. For semen stored at a range of volumes as pellets frozen on dry ice (0.2 to 2.0 mL) or straws frozen in liquid nitrogen vapor (0.25 to 0.5 mL) and thawed in a waterbath at 20°C, the post-thawing motilities were similar even though the patterns of cooling and thawing differed markedly between methods of freezing and sizes of pellets and straws. (C) 2000 by Elsevier Science Inc.
History
Publication title
TheriogenologyVolume
54Pagination
467-480ISSN
0093-691XDepartment/School
Institute for Marine and Antarctic StudiesPublisher
ElsevierPlace of publication
NetherlandsRepository Status
- Restricted