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Sperm survival during short-term storage and after cryopreservation of semen from striped trumpeter ( Latris lineata)

Citation

Ritar, AJ and Campet, M, Sperm survival during short-term storage and after cryopreservation of semen from striped trumpeter ( Latris lineata), Theriogenology, 54, (3) pp. 467-480. ISSN 0093-691X (2000) [Refereed Article]

DOI: doi:10.1016/S0093-691X(00)00363-0

Abstract

Methods of short-term storage and cryopreservation were examined for semen from striped trumpeter (Latris lineata). For fresh semen at 18°C, the percentage of motile sperm declined rapidly from over 80% immediately after activation with sea water to less than 2% within 9 min after activation. The motility after activation of undiluted fresh sperm stored at 5°C was maintained for two days and then declined markedly so that by the eighth day, sperm were mostly immotile after activation. The post-thawing motility was higher for sperm frozen with a non-activating diluent containing 2.84 M DMSO in saline (117 mM NaCl) than in an activating glycerol (2 M) medium in dilute sea water (300 mOsm). Post-thawing motility was higher for a dilution rate of 1:5 (semen:diluent) than 1:2 or 1:11 but was similar when frozen semen was thawed at 10°, 20°or 30°C. For semen stored at a range of volumes as pellets frozen on dry ice (0.2 to 2.0 mL) or straws frozen in liquid nitrogen vapor (0.25 to 0.5 mL) and thawed in a waterbath at 20°C, the post-thawing motilities were similar even though the patterns of cooling and thawing differed markedly between methods of freezing and sizes of pellets and straws. (C) 2000 by Elsevier Science Inc.

Item Details

Item Type:Refereed Article
Research Division:Agricultural and Veterinary Sciences
Research Group:Fisheries Sciences
Research Field:Aquaculture
Objective Division:Animal Production and Animal Primary Products
Objective Group:Fisheries - Aquaculture
Objective Field:Fisheries - Aquaculture not elsewhere classified
Author:Ritar, AJ (Associate Professor Arthur Ritar)
ID Code:19441
Year Published:2000
Web of Science® Times Cited:8
Deposited By:TAFI - Marine Research Laboratory
Deposited On:2000-08-01
Last Modified:2008-09-17
Downloads:0

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