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Elution mechanism in electrostatic ion chromatography with histidine as an isoelectric ampholytic mobile phase

Citation

Macka, M and Haddad, PR, Elution mechanism in electrostatic ion chromatography with histidine as an isoelectric ampholytic mobile phase, J. Chromatogr. A, 884, (1-2) pp. 287-296. ISSN 0021-9673 (2000) [Refereed Article]

DOI: doi:10.1016/S0021-9673(00)00167-9

Abstract

Electrostatic ion chromatography (EIC) using a zwitterionic stationary phase (formed by coating a C18 material with a hydrophobic zwitterionic surfactant) was studied with a mobile phase comprising an aqueous solution of histidine at the pH of its isoelectric point, together with non-suppressed conductometric detection. Anions and cations were found to be eluted as separate peaks, unlike the elution behaviour observed on the same system when pure water was used as mobile phase. An explanation was suggested in terms of protonation equilibria of the overall uncharged histidine to form small amounts of histidine cations and anions in the mobile phase which could act as counterions for analyte anions and cations. This suggestion was supported by measured pH changes occurring in the bands of eluted analyte anions (a decreased pH compared to the mobile phase) and cations (an increased pH compared to the mobile phase). The analytical potential of this type of EIC is discussed. Copyright (C) 2000 Elsevier Science B.V.

Item Details

Item Type:Refereed Article
Research Division:Chemical Sciences
Research Group:Analytical Chemistry
Research Field:Separation Science
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Chemical Sciences
Author:Macka, M (Professor Mirek Macka)
Author:Haddad, PR (Professor Paul Haddad)
ID Code:19328
Year Published:2000
Web of Science® Times Cited:7
Deposited By:Chemistry
Deposited On:2000-08-01
Last Modified:2011-08-04
Downloads:0

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