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Modelling the combined temperature and salt (NaCl) limits for growth of a pathogenic Escherichia coli strain using nonlinear logistic regression

Citation

Salter, MA and Ratkowsky, DA and Ross, T and McMeekin, TA, Modelling the combined temperature and salt (NaCl) limits for growth of a pathogenic Escherichia coli strain using nonlinear logistic regression, International Journal of Food Microbiology, 61, (2-3) pp. 159-167. ISSN 0168-1605 (2000) [Refereed Article]

DOI: doi:10.1016/S0168-1605(00)00352-4

Abstract

A broth-based method is used to determine if exponential phase Escherichia coli R31, an STEC, is able to grow within 50 days under various combinations of sub-optimal temperatures and salt concentrations. From these data, the growth limits for combinations of temperature (7.7-37.0°C) and water activity (0.943-0.987; NaCl as humectant) are defined and modelled using a nonlinear logistic regression model. That form of model is able to predict the combinations of salt concentration/water activity and temperature that will prevent the growth of E. coli R31 with selected levels of confidence. The model fitted the data with an approximate concordance rate of 97.3%. The minimum water activity that permitted growth occurred in the range 25-30°C, the temperature range which optimises cell yield. At temperatures below this range the minimum water activity which allowed growth increased with decreasing temperature.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Microbiology
Research Field:Microbiology not elsewhere classified
Objective Division:Health
Objective Group:Public Health (excl. Specific Population Health)
Objective Field:Food Safety
Author:Salter, MA (Mr Salter)
Author:Ratkowsky, DA (Dr David Ratkowsky)
Author:Ross, T (Associate Professor Tom Ross)
Author:McMeekin, TA (Professor Thomas McMeekin)
ID Code:19085
Year Published:2000
Web of Science® Times Cited:59
Deposited By:Agricultural Science
Deposited On:2000-08-01
Last Modified:2014-05-28
Downloads:0

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