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Effect of Sudden Salt Stress on Ion Fluxes in Intact Wheat Suspension Cells

Citation

Babourina, OK and Leonova, T and Shabala, SN and Newman, IA, Effect of Sudden Salt Stress on Ion Fluxes in Intact Wheat Suspension Cells, Annals of Botany, 85, (6) pp. 759-767. ISSN 0305-7364 (2000) [Refereed Article]

DOI: doi:10.1006/anbo.2000.1136

Abstract

Although salinity is one of the major problems limiting agricultural production around the world, the underlying mechanisms of high NaCl perception and tolerance are still poorly understood. The effects of different bathing solutions and fusicoccin (FC), a known activator of plasma membrane ATPase, on plasma membrane potential (E(m)) and net fluxes of Na+, K+ and H+ were studied in wheat suspension cells (Triticum aestivum) in response to different NaCl treatments. E(m) of cells in Murashige and Skoog (MS) medium was less negative than in cells exposed to a medium containing 10 mM KCl + 0.1 mM CaCl2 (KSM) and to a basic salt medium (BSM), containing 1 mM KCl and 0.1 mM CaCl2. Multiphasic Na+ accumulation in cells was observed, peaking at 13 min after addition of 120 mM NaCl to MS medium. This time scale was in good agreement with net Na+ flux changes measured non-invasively by moving ion-selective microelectrodes (the MIFE system). When 120 mM NaCl was added to all media studied, a quick rise of Na+ influx was reversed within the first 20 min. In both 120 and 20 mM NaCl treatments in MS medium, net Na+ efflux was observed, indicating that active Na+ transporters function in the plant cell response to salt stress. Lower external K+ concentrations (KSM and BSM) and FC pre-treatment caused shifts in Na+ fluxes towards net influx at 120 mM NaCl stress. (C) 2000 Annals of Botany Company.

Item Details

Item Type:Refereed Article
Research Division:Agricultural and Veterinary Sciences
Research Group:Crop and Pasture Production
Research Field:Crop and Pasture Nutrition
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Environmental Sciences
Author:Babourina, OK (Dr Olga Babourina)
Author:Shabala, SN (Professor Sergey Shabala)
Author:Newman, IA (Dr Ian Newman)
ID Code:18791
Year Published:2000
Web of Science® Times Cited:18
Deposited By:Agricultural Science
Deposited On:2000-08-01
Last Modified:2011-09-20
Downloads:0

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