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Verapamil-induced kinetics of ion flux in oat seedlings


Babourina, OK and Shabala, SN and Newman, IA, Verapamil-induced kinetics of ion flux in oat seedlings, Australian Journal of Plant Physiology, 27, (11) pp. 1031-1040. ISSN 0310-7841 (2000) [Refereed Article]

DOI: doi:10.1071/PP99182


Verapamil application in ion transport studies on plant cells is widespread; however, the mechanism of its action is still poorly understood. Net flux of Ca2+, K+, Na+, H+ and Cl- were measured in solution around oat seedlings using the non-invasive ion-selective microelectrode MIFE technique. The verapamil effect on intact plant tissues was a distinct immediate influx of monovalent cations, H+, K+ or Na+. Pre-treatment with tetraethylammonium, Cs+, Ba2+ or Ca2+ did not affect K+ flux changes, whereas DMSO, Cd2+ and vanadate increased verapamil-induced K+ influx. Verapamil-induced K+ flux increased with increased external KCl concentration or pH. Verapamil concentration up to 1 mm failed to shift Ca2+ flux to efflux. Pre-treatment with adrenaline and dopamine agonists and antagonists led to changes in verapamil-induced ion flux, especially for Ca2+. We suggest that a transporter that allows entry of K+ and Na+ was the main system providing monovalent ion influx after verapamil application.

Item Details

Item Type:Refereed Article
Research Division:Agricultural, Veterinary and Food Sciences
Research Group:Crop and pasture production
Research Field:Crop and pasture biochemistry and physiology
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the environmental sciences
UTAS Author:Babourina, OK (Dr Olga Babourina)
UTAS Author:Shabala, SN (Professor Sergey Shabala)
UTAS Author:Newman, IA (Dr Ian Newman)
ID Code:18790
Year Published:2000
Web of Science® Times Cited:14
Deposited By:Agricultural Science
Deposited On:2000-08-01
Last Modified:2011-09-26

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