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Verapamil-induced kinetics of ion flux in oat seedlings
journal contribution
posted on 2023-05-16, 12:05 authored by Babourina, OK, Sergey ShabalaSergey Shabala, Ian NewmanIan NewmanVerapamil application in ion transport studies on plant cells is widespread; however, the mechanism of its action is still poorly understood. Net flux of Ca2+, K+, Na+, H+ and Cl- were measured in solution around oat seedlings using the non-invasive ion-selective microelectrode MIFE technique. The verapamil effect on intact plant tissues was a distinct immediate influx of monovalent cations, H+, K+ or Na+. Pre-treatment with tetraethylammonium, Cs+, Ba2+ or Ca2+ did not affect K+ flux changes, whereas DMSO, Cd2+ and vanadate increased verapamil-induced K+ influx. Verapamil-induced K+ flux increased with increased external KCl concentration or pH. Verapamil concentration up to 1 mm failed to shift Ca2+ flux to efflux. Pre-treatment with adrenaline and dopamine agonists and antagonists led to changes in verapamil-induced ion flux, especially for Ca2+. We suggest that a transporter that allows entry of K+ and Na+ was the main system providing monovalent ion influx after verapamil application.
History
Publication title
Australian Journal of Plant PhysiologyVolume
27Issue
11Pagination
1031-1040ISSN
0310-7841Department/School
Tasmanian Institute of Agriculture (TIA)Publisher
CSIRO PublishingPlace of publication
Collingwood, AustraliaRepository Status
- Restricted