eCite Digital Repository

Salinity Effects on the Activity of Plasma Membrane H+ and Ca2+ Transporters in Bean Leaf Mesophyll: Masking Role of the Cell Wall

Citation

Shabala, SN and Newman, IA, Salinity Effects on the Activity of Plasma Membrane H+ and Ca2+ Transporters in Bean Leaf Mesophyll: Masking Role of the Cell Wall, Annals of Botany, 85, (5) pp. 681-686. ISSN 0305-7364 (2000) [Refereed Article]

DOI: doi:10.1006/anbo.2000.1131

Abstract

Net fluxes of H+ and Ca2+ were measured in the mesophyll tissue of broad bean (Vicia faba L.) leaves and in protoplasts derived from these cells. NaCl at 90 mm enhanced H+ extrusion in both protoplasts and tissue, but in different ways. Proton extrusion was inhibited by vanadate, suggesting the involvement of the plasma membrane H+-ATPase in cell responses to salinity. There was virtually no effect of NaCl on the net Ca2+ flux in protoplasts, while in the tissue a large transient Ca2+ efflux followed the salt treatment. Salt-induced Ca2+ efflux was essentially independent of external Ca2+ concentrations in the range 0.1 to 10 mm. Also, Ca2+ flux responses were 'saturated' above 50 mM NaCl. It is suggested that almost all the measured Ca2+ flux originates from Na+/Ca2+ and H+/Ca2+ ion exchange in the cell wall. This conclusion was supported by the results of modelling cation exchange in the cell wall. (C) 2000 Annals of Botany Company.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Biochemistry and Cell Biology
Research Field:Receptors and Membrane Biology
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Environmental Sciences
Author:Shabala, SN (Professor Sergey Shabala)
Author:Newman, IA (Dr Ian Newman)
ID Code:18786
Year Published:2000
Web of Science® Times Cited:45
Deposited By:Agricultural Science
Deposited On:2000-08-01
Last Modified:2011-09-20
Downloads:0

Repository Staff Only: item control page