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Ion-specific mechanisms of osmoregulation in bean mesophyll cells

Citation

Shabala, SN and Babourina, OK and Newman, IA, Ion-specific mechanisms of osmoregulation in bean mesophyll cells, Journal of Experimental Botany, 51, (348) pp. 1243-1253. ISSN 0022-0957 (2000) [Refereed Article]

DOI: doi:10.1093/jexbot/51.348.1243

Abstract

Transient kinetics of net H+,K+, Ca2+, and Cl- fluxes were measured non-invasively, using an ion-selective microelectrode technique, for bean (Vicia faba L.) leaf mesophyll in response to 150 mM mannitol treatment. In a parallel set of experiments, changes in the plasma membrane potential and the total proline content in leaves were monitored. Regardless of the ionic composition of the bath solution, hyperosmotic stress caused a significant increase in the K+ and Cl- uptake into mesophyll cells. At the same time, no significant proline changes were observed for at least 16 h after the onset of stress. Experiments with inhibitors suggested that potassium inward rectifier (KIR) channels, exhibiting mechanosensitive properties and acting as primary receptors of osmotic stress, are likely to be involved. Due to the coupling by membrane potential, changes in K+ and Cl- transport may modify activity of the plasma membrane H+-pump. Such coupling may also be responsible for the mannitol-induced oscillations (period of about 4 min) in net ion fluxes observed in 90% of plants. Calculations show that influx of K+ and Cl- observed in response to hyperosmotic treatment may provide an adequate osmotic adjustment in bean mesophyll, which suggests that the activity of the plasma membrane transporters for these ions should be targeted to improve osmotolerance, at least in this crop.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Plant Biology
Research Field:Plant Pathology
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Environmental Sciences
Author:Shabala, SN (Professor Sergey Shabala)
Author:Babourina, OK (Dr Olga Babourina)
Author:Newman, IA (Dr Ian Newman)
ID Code:18785
Year Published:2000
Web of Science® Times Cited:65
Deposited By:Agricultural Science
Deposited On:2000-08-01
Last Modified:2001-03-20
Downloads:0

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