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Genetic variation among strains of the toxic dinoflagellate Gymnodinium catenatum (Dinophyceae)


Bolch, CJS and Blackburn, SI and Hallegraeff, GM and Vaillancourt, RE, Genetic variation among strains of the toxic dinoflagellate Gymnodinium catenatum (Dinophyceae), Journal of Phycology, 35, (2) pp. 356-367. ISSN 0022-3646 (1999) [Refereed Article]

DOI: doi:10.1046/j.1529-8817.1999.3520356.x


The toxic dinoflagellate Gymnodinium carenatum Graham has formed recurrent toxic blooms in southeastern Tasmanian waters since its discovery in the area in 1986. Current evidence suggests that this species might have been introduced to Tasmania prior to 1973, possibly in cargo vessel ballast water carried from populations in Japan or Spain, followed by recent dispersal to mainland Australia. To examine this hypothesis, cultured strains from G. catenatum populations in Australia, Spain, Portugal, and Japan were examined using allozymes and randomly amplified polymorphic DNA (RAPD). Allozyme screening detected very limited polymorphism and was not useful for population comparisons; however, Australian, Spanish, Portuguese, and Japanese strains showed considerable RAPD diversity, and all strains examined represented unique genotypes. Multidimensional scaling analysis (MDS) of RAPD genetic distances between strains showed clear separation of strains into three nonoverlapping regional clusters: Australia, Japan, and Spain/Portugal. Analysis of genetic distances between strains from the three regional populations indicated that Australian strains were almost equally related to both the Spanish/Portuguese population and the Japanese population. Analysis of molecular variance (AMOVA) found that genetic variation was partitioned mainly within populations (87%) compared to the variation between the regions (8%) and between populations within regions (5%). The potential source population for Tasmania's introduced G. catenatum remains equivocal; however, strains from the recently discovered mainland Australian population (Port Lincoln, South Australia, 1996) clustered with Tasmanian strains, supporting the notion of a secondary relocation of Tasmanian G. catenatum populations to the mainland via a shipping vector. Geographic and temporal clustering of strains was evident among the Tasmanian strains, indicating that genetic exchange between neighboring estuaries is limited and that Tasmanian G. catenatum blooms are composed of localized, estuary-bound subpopulations.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Plant biology
Research Field:Phycology (incl. marine grasses)
Objective Division:Environmental Management
Objective Group:Coastal and estuarine systems and management
Objective Field:Control of pests, diseases and exotic species in coastal and estuarine environments
UTAS Author:Bolch, CJS (Associate Professor Christopher Bolch)
UTAS Author:Hallegraeff, GM (Professor Gustaaf Hallegraeff)
UTAS Author:Vaillancourt, RE (Professor Rene Vaillancourt)
ID Code:17953
Year Published:1999
Web of Science® Times Cited:62
Deposited By:Plant Science
Deposited On:1999-08-01
Last Modified:2011-08-05

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