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LCS, the Chlamydomonas Homologue of the t complex-encoded Protein Tctex2, is Essential for Outer Dynein Arm Assembly

Citation

Pazour, GJ and Koutoulis, A and Benashski, SE and Dickert, BL and Sheng, S and Patel-King, RS and King, SM and Witman, GB, LCS, the Chlamydomonas Homologue of the t complex-encoded Protein Tctex2, is Essential for Outer Dynein Arm Assembly, Molecular Biology of the Cell, 10, (10) pp. 3507-3520. ISSN 1059-1524 (1999) [Refereed Article]

DOI: doi:10.1091/mbc.10.10.3507

Abstract

Tctex2 is thought to be one of the distorter genes of the mouse t haplotype. This complex greatly biases the segregation of the chromosome that carries it such that in heterozygous +/t males, the t haplotype is transmitted to >95% of the offspring, a phenomenon known as transmission ratio distortion. The LC2 outer dynein arm light chain of Chlamydomonas reinhardtii is a homologue of the mouse protein Tctex2. We have identified Chlamydomonas insertional mutants with deletions in the gene encoding LC2 and demonstrate that the LC2 gene is the same as the ODA12 gene, the product of which had not been identified previously. Complete deletion of the LC2/ODA12 gene causes loss of all outer arms and a slow jerky swimming phenotype. Transformation of the deletion mutant with the cloned LC2/ODA12 gene restores the outer arms and rescues the motility phenotype. Therefore, LC2 is required for outer arm assembly. The fact that LC2 is an essential subunit of flagellar outer dynein arms allows us to propose a detailed mechanism whereby transmission ratio distortion is explained by the differential binding of mutant (t haplotype encoded) and wild-type dyneins to the axonemal microtubules of t-bearing or wildtype sperm, with resulting differences in their motility.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Genetics
Research Field:Gene Expression (incl. Microarray and other genome-wide approaches)
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Reproductive System and Disorders
Author:Koutoulis, A (Professor Anthony Koutoulis)
ID Code:17552
Year Published:1999
Web of Science® Times Cited:41
Deposited By:Plant Science
Deposited On:1999-08-01
Last Modified:2000-05-22
Downloads:0

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