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The influence of the null le-2 mutation on gibberellin levels in developing pea seeds


Lester, DR and Mackenzie-Hose, AK and Davies, PJ and Ross, JJ and Reid, JB, The influence of the null le-2 mutation on gibberellin levels in developing pea seeds, Plant Growth Regulation, 27, (2) pp. 83-89. ISSN 0167-6903 (1999) [Refereed Article]

DOI: doi:10.1023/A:1006158701016


The Le gene of pea encodes a gibberellin 3β-hydroxylase. Heterologous expression of the le-2 allele indicated that a truncated protein was produced, confirming that le-2 is a null mutation. The Le expression product was unable to metabolise GA 12, but was able to produce a small quantity of GAs from GA 29. The le-2 mutation had no effect on the levels of GA 1, GA 4 or GA 8 in developing seeds. Measurements of mRNA levels indicate that the Le gene is only weakly expressed in young pea seeds. These results explain why mutant alleles at the Le locus have no major impact on seed development, even though 3β-hydroxylated GAs are essential for normal seed development in pea. Rather, a second 3β-hydroxylase, with a different substrate specificity, may be expressed in young seeds, resulting in a different biosynthetic pathway leading to the biologically active GAs.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Plant biology
Research Field:Plant physiology
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the environmental sciences
UTAS Author:Lester, DR (Dr Diane Lester)
UTAS Author:Mackenzie-Hose, AK (Mr Alasdair Kenneth Mackenzie-Hose)
UTAS Author:Davies, PJ (Dr Davies)
UTAS Author:Ross, JJ (Associate Professor John Ross)
UTAS Author:Reid, JB (Professor Jim Reid)
ID Code:17522
Year Published:1999
Web of Science® Times Cited:14
Deposited By:Plant Science
Deposited On:1999-08-01
Last Modified:2010-11-09

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