eCite Digital Repository

Abscisic Acid levels in seeds of the giberellin-deficient mutant lh-2 of pea ( Pisum Sativum)

Citation

Batge, SL and Ross, JJ and Reid, JB, Abscisic Acid levels in seeds of the giberellin-deficient mutant lh-2 of pea ( Pisum Sativum), Physiologia Plantarum, 105, (3) pp. 485-490. ISSN 0031-9317 (1999) [Refereed Article]

DOI: doi:10.1034/j.1399-3054.1999.105313.x

Abstract

The lh-2 mutation in garden pea (Pisum sativum L.) blocks an early step in the gibberellin (GA) biosynthesis pathway, the three-step oxidation of ent-kaurene to ent-kaurenoic acid. As a result, only low levels of GAs, including the bioactive GA1, are found in shoots and seeds of lh-2 plants. Mutant plants are dwarf in stature, and show increased seed abortion and decreased seed weight, compared with seeds of the tall wild-type (WT) progenitor (cv. Torsdag). The aberrant seed development of lh-2 plants is associated with reduced levels of GA1 and GA3, and with an accumulation of abscisic acid (ABA) in young seeds (pre-contact point). This ABA accumulation is typically 3- to 4-fold, and can be up to 6-fold, compared with control plants. To investigate whether the accumulation of ABA is partly responsible for causing the observed seed abortion in lh-2 plants, we constructed a double mutant between the lh-2 allele and wil. The wil mutation blocks ABA biosynthesis, and reduces ABA levels in young seeds by 10-fold. Introduction of the wil mutation reduces the endogenous ABA levels in young lh-2 seeds, but fails to rescue the seeds from abortion. This indicates that the effects of lh-2 on seed development are not mediated through increased ABA levels, and is consistent with previous evidence that GAs are the controlling factor underlying the lh-2 seed phenotype in pea.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Plant Biology
Research Field:Plant Physiology
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Environmental Sciences
Author:Batge, SL (Ms Shona Lee Batge)
Author:Ross, JJ (Associate Professor John Ross)
Author:Reid, JB (Professor Jim Reid)
ID Code:17495
Year Published:1999
Web of Science® Times Cited:18
Deposited By:Plant Science
Deposited On:1999-08-01
Last Modified:2010-11-05
Downloads:0

Repository Staff Only: item control page