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Simple gill Smear Staining for Diagnosis of Amoebic Gill Disease


Zilberg, D and Nowak, BF and Carson, J and Wagner, T, Simple gill Smear Staining for Diagnosis of Amoebic Gill Disease, Bulletin of European Association of Fish Pathologists, 19, (5) pp. 186-189. ISSN 0108-0288 (1999) [Refereed Article]


Amoebic gill disease (AGD) caused by a Paramoeba spp. is the major disease affecting cultured Atlantic salmon in Tasmania. Primary diagnosis of the disease on the farm relies on gross morphology, and it is then confirmed in the laboratory with an immunofluorescence antibody test (IFAT). In this study we evaluated the potential use of a rapid method for the detection of AGD using a Quick Dip* stain and compared it to the established IFAT. Quick Dip* staining revealed 96% agreement with IFAT in the determination whether a sample is positive or negative for AGD, with sensitivity and specificity values of 88.23% and 92.85%, respectively and a kappa value of 0.7628. Mean number of Paramoeba spp. per field of view positively correlated in the two methods (r=0.972), although it was significantly greater when IFAT was used. Quick Dip* stained Paramoeba spp. in a mucus smear is identified by its morphological features. It measures 15 to 20 μm, appearing dark blue with a darker blue and purple-stained internal organelles. The study suggests that Quick Dip* can be used as a fast method for the diagnosis of AGD.

Item Details

Item Type:Refereed Article
Research Division:Agricultural, Veterinary and Food Sciences
Research Group:Fisheries sciences
Research Field:Fish pests and diseases
Objective Division:Animal Production and Animal Primary Products
Objective Group:Fisheries - aquaculture
Objective Field:Fisheries - aquaculture not elsewhere classified
UTAS Author:Zilberg, D (Dr Dina Zilberg)
UTAS Author:Nowak, BF (Professor Barbara Nowak)
ID Code:16605
Year Published:1999
Web of Science® Times Cited:17
Deposited By:TAFI - Aquaculture
Deposited On:1999-08-01
Last Modified:2000-05-09

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