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Evaluation of Simple Instruments for the Measurement of Blood Glucose and Lactate, and Plasma Protein as Stress Indicators in Fish

Citation

Wells, RMG and Pankhurst, NW, Evaluation of Simple Instruments for the Measurement of Blood Glucose and Lactate, and Plasma Protein as Stress Indicators in Fish, Journal of the World Aquaculture Society, 30, (2) pp. 276-284. ISSN 0893-8849 (1999) [Refereed Article]

DOI: doi:10.1111/j.1749-7345.1999.tb00876.x

Abstract

Responses of rainbow trout, Oncorhynchus mykiss, to handling and confinement stress were used to validate the efficacy of portable instruments for measuring blood glucose and lactate, and plasma protein. Glucose and lactate increased with time and showed good linear correlation with time after disturbance. The Advantage™ blood glucose and Accusport™ lactate meters were similarly correlated, except that values were consistently lower than those obtained by established laboratory techniques. Refractometry gave higher plasma protein values than results from biuret reagent but with excellent correlation. These findings indicate a potential role for portable instruments in field and hatchery locations where relative rather than absolute values may be used to evaluate responses to stressors.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Physiology
Research Field:Comparative Physiology
Objective Division:Animal Production and Animal Primary Products
Objective Group:Fisheries - Aquaculture
Objective Field:Fisheries - Aquaculture not elsewhere classified
Author:Pankhurst, NW (Professor Ned Pankhurst)
ID Code:16571
Year Published:1999
Web of Science® Times Cited:67
Deposited By:TAFI - Aquaculture
Deposited On:1999-08-01
Last Modified:2000-06-06
Downloads:0

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