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Capillary electrophoresis with end-capillary potentiometric detection using a copper electrode


Macka, M and Gerhardt, G and Andersson, PE and Bogan, DP and Cassidy, RM and Haddad, PR, Capillary electrophoresis with end-capillary potentiometric detection using a copper electrode, Electrophoresis, 20, (12) pp. 2539-2546. ISSN 0173-0835 (1999) [Refereed Article]

DOI: doi:10.1002/(SICI)1522-2683(19990801)20:12<2539::AID-ELPS2539>3.0.CO;2-0


Potentiometric end-capillary detection in capillary electrophoresis has the advantage of relatively easy miniaturisation without having to compromise the concentration sensitivity. Potentiometric end-capillary detection using a copper electrode is also attractive because of the sensitive detection of many inorganic and organic UV-transparent ions and the ability to work in both direct and indirect mode. In this work, detection of a number of common anions in a tartrate electrolyte at pH 3 was studied. The influence of the end-capillary detection geometry on the detection performance was investigated. An end-capillary detection cell allowing the separation capillary to be changed without the need to realign the detection electrode was constructed and fitted into a commercial CE apparatus. Under the optimal configuration, which was a 25 μm diameter copper electrode aligned coaxially with a 25 μm capillary and positioned at a distance of about 25 μm from the capillary end, excellent peak shapes were achieved and comparison with simultaneous on-capillary photometric detection showed no additional peak broadening. Good sensitivity was obtained, resulting in concentration limits of detection (LODs) in the low μM range and mass LODs in the low amol range. Examples of separations of inorganic and organic anions are presented and the analytical potential of the detection method is assessed.

Item Details

Item Type:Refereed Article
Research Division:Chemical Sciences
Research Group:Analytical chemistry
Research Field:Separation science
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the chemical sciences
UTAS Author:Macka, M (Professor Mirek Macka)
UTAS Author:Gerhardt, G (Dr Geoff Gerhardt)
UTAS Author:Andersson, PE (Dr Andersson)
UTAS Author:Bogan, DP (Dr Declan Bogan)
UTAS Author:Cassidy, RM (Professor Richard Cassidy)
UTAS Author:Haddad, PR (Professor Paul Haddad)
ID Code:16244
Year Published:1999
Web of Science® Times Cited:17
Deposited By:Chemistry
Deposited On:1999-08-01
Last Modified:2011-08-04

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