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Retention modelling of electrostatic and adsorption effects of aliphatic and aromatic carboxylic acids in ion-exclusion chromatography

Citation

Ng, KL and Paull, B and Haddad, PR and Tanaka, K, Retention modelling of electrostatic and adsorption effects of aliphatic and aromatic carboxylic acids in ion-exclusion chromatography, Journal of Chromatography A, 850, (1-2) pp. 17-27. ISSN 0021-9673 (1999) [Refereed Article]

DOI: doi:10.1016/S0021-9673(99)00735-9

Abstract

The retention mechanism of aliphatic and aromatic carboxylic acids in ion-exclusion chromatography has been investigated with consideration of simultaneous electrostatic repulsion effects and hydrophobic adsorption effects. A mathematical relationship between the retention factor of the analyte and the mobile-phase composition (sulfuric acid concentration and percentage of methanol), the type of analyte (pK(a) and hydrophobicity) and some physical characteristics of the stationary phase has been derived. Thirteen carboxylic acids (comprising mono- and divalent, aliphatic and aromatic acids) were chosen and used to acquire retention data on three different cation-exchange stationary phases (in which the sulfonate functional groups are bound to polystyrene-divinylbenzene, polymethacrylate or silica) using 14 mobile-phase compositions of varying pH and percent methanol. These retention data were used to derive the parameters necessary to solve the retention model using non-linear regression. In this way, a quantitative measure of the effects of adsorption phenomena on analyte retention were obtained. The model was then used to optimise the separation of nine carboxylic acids. Copyright (C) 1999.

Item Details

Item Type:Refereed Article
Research Division:Chemical Sciences
Research Group:Analytical Chemistry
Research Field:Separation Science
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Chemical Sciences
Author:Ng, KL (Miss Kai Ng)
Author:Paull, B (Professor Brett Paull)
Author:Haddad, PR (Professor Paul Haddad)
Author:Tanaka, K (Dr Kazuhiko Tanaka)
ID Code:16242
Year Published:1999
Web of Science® Times Cited:34
Deposited By:Chemistry
Deposited On:1999-08-01
Last Modified:2011-08-04
Downloads:0

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