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Theoretical migration model for micellar capillary electrophoresis and its application to the separation of anionic metal complexes of HEDTC and CDTA

Citation

Breadmore, MC and Macka, M and Haddad, PR, Theoretical migration model for micellar capillary electrophoresis and its application to the separation of anionic metal complexes of HEDTC and CDTA, Analytical Chemistry, 71, (9) pp. 1826-1833. ISSN 0003-2700 (1999) [Refereed Article]

DOI: doi:10.1021/ac981044h

Abstract

A mathematical model relating the effective mobility of an analyte in micellar capillary electrophoresis (MCE) to the concentration of surfactant and organic modifier in the background electrolyte (BGE) was derived. Effective mobility is expressed in terms of the electrophoretic mobility of the analyte, the partition coefficient of the analyte into the micelle, and the influence of organic modifier on these two factors. The performance of the model was evaluated using Cd(II), Pb(II), Co(II), Ni(II), Bi(III), Cu(II), and Hg(II) complexes of bis(2-hydroxyethyl) dithiocarbamate, all of which carry a partial negative charge, and Cd(II), Pb(II), Co(II), Ni(II), Bi(III), Cu(II), Hg(II), Fe(III), Ag(I), Tl(I), and Mn(II) complexes of trans-1,2-diaminocyclohexane-N,N,N',N'-tetraacetic acid, all of which are anionic having charges in the range -1 to -3. These analytes were separated in borate BGEs containing 10-50 mM sodium dodecyl sulfate and 0-20% (v/v) methanol. Nonlinear regression was used to derive parameters for the model from experimental data and these parameters were used to predict effective mobilities of the analytes. Predicted values of effective mobilities agreed with experimental values to within 3.1%. Values of parameters from the model equation are used to explain changes in separation selectivity observed at different BGE compositions and the model equation is shown to be applicable to computer-assisted optimization of the BGE composition, in MCE using a limited number of experiments.

Item Details

Item Type:Refereed Article
Research Division:Chemical Sciences
Research Group:Analytical Chemistry
Research Field:Separation Science
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Chemical Sciences
Author:Breadmore, MC (Professor Michael Breadmore)
Author:Macka, M (Professor Mirek Macka)
Author:Haddad, PR (Professor Paul Haddad)
ID Code:16206
Year Published:1999
Web of Science® Times Cited:17
Deposited By:Chemistry
Deposited On:1999-08-01
Last Modified:2009-11-19
Downloads:0

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