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Artificial insemination with cryopreserved semen from striped trumpeter (Latris lineata)


Ritar, AJ, Artificial insemination with cryopreserved semen from striped trumpeter (Latris lineata), Aquaculture, 180, (1-2) pp. 177-187. ISSN 0044-8486 (1999) [Refereed Article]

DOI: doi:10.1016/S0044-8486(99)00109-X


Semen from striped trumpeter (ST) (Latris lineata) was frozen either as pellets on dry ice or in straws in the vapour of liquid nitrogen and then stored in liquid nitrogen until used for insemination. The motility of thawed semen was invariably lower than for fresh semen. ST eggs (collected from females treated with cholesterol implants containing LHRHa) were inseminated with fresh and frozen-thawed semen before incubation at 12-13°C in sterile filtered sea water with regular water exchanges until larval hatch (approximately 156 h after insemination). Fertilisation and larval hatch rates were: (1) mostly lower for frozen-thawed than for fresh semen; (2) higher for semen frozen with a diluent containing DMSO than containing glycerol; and (3) similar for semen cryopreserved for 1 year to recently frozen semen. The fertilisation rates were similar for fresh semen and semen frozen in 0.25 ml straws (79.6 ± 2.7% and 75.2 ± 1.6%) but lower for semen frozen in 0.5 ml straws and as 0.25 and 2.0 ml pellets (69.5 ± 2.8%, 67.3 ± 1.5% and 69.6 ± 5.3%, respectively; P < 0.05). Egg mortalities were highest on days 0 and 2 after insemination and were low thereafter until hatch regardless of type of semen. The rates of larval hatch for semen frozen in 0.25 and 0.5 ml straws (44.3 ± 2.9% and 44.2 ± 2.0%) were higher than for semen frozen in 0.25 and 2.0 ml pellets (29.2 ± 1.7% and 33.3 ± 3.9%; P < 0.05) but all were lower than for fresh semen (60.5 ± 1.5%). When eggs from banded morwong (BMW, Cheilodactylus spectabilis) were inseminated with cryopreserved ST semen, the fertilisation and hatch rates were lower (40.8 ± 5.6% and 7.6 ± 2.1%) than when inseminated with fresh BMW semen (68.4 ± 8.8% and 25.8 ± 6.9%). Embryonic development until hatch appeared to be normal for hybrid embryos. Two days after hatch, hybrid larvae appeared more like those from ST (i.e., possible paternal influence on larvae) than from BMW.

Item Details

Item Type:Refereed Article
Research Division:Agricultural, Veterinary and Food Sciences
Research Group:Fisheries sciences
Research Field:Aquaculture
Objective Division:Animal Production and Animal Primary Products
Objective Group:Fisheries - aquaculture
Objective Field:Fisheries - aquaculture not elsewhere classified
UTAS Author:Ritar, AJ (Associate Professor Arthur Ritar)
ID Code:16178
Year Published:1999
Web of Science® Times Cited:19
Deposited By:TAFI - Marine Research Laboratory
Deposited On:1999-08-01
Last Modified:2011-08-04

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