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Comparative metabolism of dietary terpene, p-cymene, in generalist and specialist folivorous marsupials

Citation

Boyle, RR and McLean, SR and Foley, W and Davies, NW, Comparative metabolism of dietary terpene, p-cymene, in generalist and specialist folivorous marsupials, Journal of Chemical Ecology, 25, (9) pp. 2109-2126. ISSN 0098-0331 (1999) [Refereed Article]


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DOI: doi:10.1023/A:1021092908058

Abstract

The urinary metabolites of the monoterpene, p-cymene, were studied in three marsupial species: a generalist herbivore, the brushtail possum (Trichosurus vulpecula), and two specialist folivores, the greater glider (Petauroides volans) and the ringtail possum (Pseudocheirus peregrinus), as well as in the laboratory rat (Rattus norvegicus). Each animal was dosed, intragastrically, with single doses of p-cymene (0.37 mmol/kg and/or 1.49 mmol/kg). Urine and feces were collected for two 24-hr periods. Quantitative analysis of urinary metabolites by extraction, gas chromatography, and mass spectrometry gave a mean recovery of 64% (range 5274%) of the administered dose in 48 hr for the four species. No fecal metabolites were found. A species-specific pattern of metabolite excretion was evident and reflected the natural occurrence of p-cymene (and terpenes in general) in the diet. If the metabolites excreted are grouped according to the total number of oxygen atoms added (one to four), then the rat excreted metabolites encompassing all degrees of oxidation, but predominantly a monooxygenated metabolite. The brushtail possum excreted metabolites having two, three, and four oxygen atoms added. The ringtail possum and greater glider only excreted metabolites with three or four oxygen atoms. Conjugation played a significant role in the excretion of oxidized metabolites in only the brushtail possums and the rat. These findings indicate that species encountering terpenes, such as p-cymene, in their normal diet have developed efficient oxidation pathways to eliminate them. This oxidative efficiency could also reduce the necessity for subsequent conjugation of metabolites which minimizes further demands on a nutritionally limited diet.

Item Details

Item Type:Refereed Article
Keywords:Trichosurus vulpecula, Pseudocheirus peregrinus, Petauroides volans, p-cymene, metabolism, detoxification, Eucalyptus, terpene
Research Division:Biological Sciences
Research Group:Other Biological Sciences
Research Field:Biological Sciences not elsewhere classified
Objective Division:Environment
Objective Group:Flora, Fauna and Biodiversity
Objective Field:Forest and Woodlands Flora, Fauna and Biodiversity
Author:Boyle, RR (Dr Rebecca Boyle)
Author:McLean, SR (Professor Stuart McLean)
Author:Davies, NW (Associate Professor Noel Davies)
ID Code:15896
Year Published:1999
Web of Science® Times Cited:60
Deposited By:Pharmacy
Deposited On:1999-08-01
Last Modified:2013-09-23
Downloads:3 View Download Statistics

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