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Divided we stand: Tracking cell proliferation with carboxyfluorescein diacetate succinimidyl ester


Lyons, AB, Divided we stand: Tracking cell proliferation with carboxyfluorescein diacetate succinimidyl ester, Immunology and Cell Biology, 77, (6) pp. 509-515. ISSN 0818-9641 (1999) [Refereed Article]

DOI: doi:10.1046/j.1440-1711.1999.00864.x


Most techniques for assessing cell division can either detect limited numbers of cell divisions (bromodeoxyuridine incorporation) or only quantify overall proliferation (tritiated thymidine incorporation). In the majority of cases, viable cells of known division history cannot subsequently be obtained for functional studies. The cells of the immune system undergo marked proliferation and differentiation during the course of an immune response. The relative lack of an organized structure of the lymphohaemopoietic system, in contrast with other organ systems, makes lineage interrelationships difficult to study. Coupled with the remarkable degree of mobility engendered by recirculation, the differentiation occurring along with cell division in the immune system has not been readily accessible for investigation. The present article reviews the development of a cell division analysis procedure based on the quantitative serial halving of the membrane permeant, stably incorporating fluorescent dye carboxyfluorescein diacetate succinimidyl ester (CFSE or CFDA, SE). The technique can be used both in vitro and in vivo, allowing eight to 10 successive divisions to be resolved by flow cytometry. Furthermore, viable cells from defined generation numbers can be sorted by flow cytometry for functional analysis.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Biochemistry and cell biology
Research Field:Cell development, proliferation and death
Objective Division:Health
Objective Group:Clinical health
Objective Field:Clinical health not elsewhere classified
UTAS Author:Lyons, AB (Associate Professor Bruce Lyons)
ID Code:15820
Year Published:1999
Web of Science® Times Cited:122
Deposited By:Pathology
Deposited On:1999-08-01
Last Modified:2000-05-26

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