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Monoxenic culture of Pratylenchus zeae on carrot discs
journal contribution
posted on 2023-05-21, 16:57 authored by Kagoda, F, Coyne, DL, James MbiruJames Mbiru, Derera, J, Tongoona, PPratylenchus zeae is widespread on maize in Uganda but studies to aid in screening cultivars for resistance to this nematode have been constrained by lack of a cheap and reliable technique for raising inoculum. Use of excised maize roots to culture P. zeae is laborious in terms of media preparation and ensuring nematode penetration of roots. Sterile carrot discs are more cost effective and relatively less laborious for rearing most root-lesion nematodes but information on their effectiveness for P. zeae is lacking. The objective of this study was to assess the efficiency of sterile carrot discs in mass culturing of P. zeae collected from maize roots. The study revealed higher reproduction rates of P. zeae on carrot discs compared to excised maize roots.
History
Publication title
Nematologia MediterraneaVolume
38Pagination
107-108ISSN
0391-9749Department/School
TSBEPublisher
Edizioni E T SPlace of publication
ItalyRepository Status
- Restricted