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Profiling Protein–DNA Interactions Cell-Type-Specifically with Targeted DamID


Marshall, OJ and Delandre, CE, Profiling Protein-DNA Interactions Cell-Type-Specifically with Targeted DamID, Methods in Molecular Biology : Chromatin Methods and Protocols, Humana Press, Julia Horsfield and Judith Marsman (ed), New York, pp. 195-213. ISBN 9781071621394 (2022) [Research Book Chapter]

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DOI: doi:10.1007/978-1-0716-2140-0


Targeted DamID (TaDa) is a means of profiling the binding of any DNA-associated protein cell-type specifically, including transcription factors, RNA polymerase and chromatin-modifying proteins. The technique is highly-sensitive, highly-reproducible, requires no mechanical disruption, cell isolation or antibody purification, and can be performed by anyone with basic molecular biology knowledge. Here, we describe the TaDa method and downstream bioinformatics data processing

Item Details

Item Type:Research Book Chapter
Keywords:Targeted DamID, Drosophila, gene expression, transcription factor binding, transcriptional
Research Division:Biological Sciences
Research Group:Genetics
Research Field:Epigenetics (incl. genome methylation and epigenomics)
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the biological sciences
UTAS Author:Marshall, OJ (Dr Owen Marshall)
UTAS Author:Delandre, CE (Dr Caroline Delandre)
ID Code:155712
Year Published:2022
Funding Support:National Health and Medical Research Council (1185220)
Deposited By:Menzies Institute for Medical Research
Deposited On:2023-03-07
Last Modified:2023-03-21

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