Profiling Protein–DNA Interactions Cell-Type-Specifically with Targeted DamID

Targeted DamID (TaDa) is a means of profiling the binding of any DNA-associated protein cell-type specifically, including transcription factors, RNA polymerase and chromatin-modifying proteins. The technique is highly-sensitive, highly-reproducible, requires no mechanical disruption, cell isolation or antibody purification, and can be performed by anyone with basic molecular biology knowledge. Here, we describe the TaDa method and downstream bioinformatics data processing