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Profiling Protein–DNA Interactions Cell-Type-Specifically with Targeted DamID
Citation
Marshall, OJ and Delandre, CE, Profiling Protein-DNA Interactions Cell-Type-Specifically with Targeted DamID, Methods in Molecular Biology : Chromatin Methods and Protocols, Humana Press, Julia Horsfield and Judith Marsman (ed), New York, pp. 195-213. ISBN 9781071621394 (2022) [Research Book Chapter]
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DOI: doi:10.1007/978-1-0716-2140-0
Abstract
Targeted DamID (TaDa) is a means of profiling the binding of any DNA-associated protein cell-type specifically, including transcription factors, RNA polymerase and chromatin-modifying proteins. The technique is highly-sensitive, highly-reproducible, requires no mechanical disruption, cell isolation or antibody purification, and can be performed by anyone with basic molecular biology knowledge. Here, we describe the TaDa method and downstream bioinformatics data processing
Item Details
| Item Type: | Research Book Chapter |
|---|---|
| Keywords: | Targeted DamID, Drosophila, gene expression, transcription factor binding, transcriptional |
| Research Division: | Biological Sciences |
| Research Group: | Genetics |
| Research Field: | Epigenetics (incl. genome methylation and epigenomics) |
| Objective Division: | Expanding Knowledge |
| Objective Group: | Expanding knowledge |
| Objective Field: | Expanding knowledge in the biological sciences |
| UTAS Author: | Marshall, OJ (Dr Owen Marshall) |
| UTAS Author: | Delandre, CE (Dr Caroline Delandre) |
| ID Code: | 155712 |
| Year Published: | 2022 |
| Funding Support: | National Health and Medical Research Council (1185220) |
| Deposited By: | Menzies Institute for Medical Research |
| Deposited On: | 2023-03-07 |
| Last Modified: | 2023-03-21 |
| Downloads: | 0 |
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