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The transcriptional regulator gene phrR in Sinorhizobium meliloti is regulated by low pH and other stresses


Reeve, WR and Tiwari, RP and Wong, CM and Dilworth, MJ and Glenn, AR, The transcriptional regulator gene phrR in Sinorhizobium meliloti is regulated by low pH and other stresses, Microbiology, 144 pp. 3335-3342. ISSN 1350-0872 (1998) [Refereed Article]

DOI: doi:10.1099/00221287-144-12-3335


The phrR gene in Sinorhizobium meliloti (previously known as Rhizobium meliloti) WSM419, directly downstream from actA, is induced by low pH or certain stresses (e.g. high concentrations of Zn 2+ , Cu 2+ , H 2 O 2 or ethanol), but not in stationary phase or by other stresses (e.g. phosphate limitation, elevated temperature, high concentrations of sucrose or iron). A DNA fragment containing the wild-type phrR gene could not be cloned and inverse PCR was therefore used to amplify a 3.5 kb BamHI fragment containing phrR from the mutant S. meliloti TG2-6 (actA::Tn5). DNA fragments from a BamHI/SalI digest of the amplified product were cloned into pUK21 and sequenced. The phrR open reading frame contiguous to actA appears to code for a 15.2 kDa protein showing significant identity with the proteins encoded by y4wC and y4aM in Rhizobium sp. NGR234. All three proteins resemble transcriptional regulators in containing a DNA-binding helix-turn-helix motif similar to that reported for URF4 in Rhodospirillum rubrum and repressors in coliphage.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Genetics
Research Field:Gene expression (incl. microarray and other genome-wide approaches)
Objective Division:Animal Production and Animal Primary Products
Objective Group:Pasture, browse and fodder crops
Objective Field:Pasture, browse and fodder crops not elsewhere classified
ID Code:15566
Year Published:1998
Web of Science® Times Cited:32
Deposited By:Agricultural Science
Deposited On:1998-08-01
Last Modified:2011-08-10

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