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Modelling growth of Bacillus cereus in paneer by one-step parameter estimation


Sarkar, D and Hunt, I and Macdonald, CA and Wang, B and Bowman, JP and Tamplin, ML, Modelling growth of Bacillus cereus in paneer by one-step parameter estimation, Food Microbiology, 112 Article 104231. ISSN 0740-0020 (2023) [Refereed Article]

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DOI: doi:10.1016/


Bacillus cereus phylogenetic group III and IV strains are commonly associated with food products and cause toxin mediated foodborne diseases. These pathogenic strains have been identified from milk and dairy products, such as reconstituted infant formula and several cheeses. Paneer is a fresh, soft cheese originating from India that is prone to foodborne pathogen contamination, such as by Bacillus cereus. However, there are no reported studies of B. cereus toxin formation in paneer or predictive models quantifying growth of the pathogen in paneer under different environmental conditions. This study assessed enterotoxin-producing potential of B. cereus group III and IV strains, isolated from dairy farm environments, in fresh paneer. Growth of a four-strain cocktail of toxin-producing B. cereus strains was measured in freshly prepared paneer incubated at 555 C and modelled using a one-step parameter estimation combined with bootstrap re-sampling to generate confidence intervals for model parameters. The pathogen grew in paneer between 10 and 50 C and the developed model fit the observed data well (R2 = 0.972, RMSE = 0.321 log10 CFU/g). The cardinal parameters for B. cereus growth in paneer along with the 95% confidence intervals were: μopt 0.812 log10 CFU/g/h (0.742, 0.917); Topt is 44.177 C (43.16, 45.49); Tmin is 4.405 C (3.973, 4.829); Tmax is 50.676 C (50.367, 51.144). The model developed can be used in food safety management plans and risk assessments to improve safety of paneer while also adding to limited information on B. cereus growth kinetics in dairy products.

Item Details

Item Type:Refereed Article
Keywords:food safety, predictive modelling, Bacillus cereus, cheese, paneer
Research Division:Agricultural, Veterinary and Food Sciences
Research Group:Food sciences
Research Field:Food safety, traceability, certification and authenticity
Objective Division:Manufacturing
Objective Group:Dairy products
Objective Field:Cheese
UTAS Author:Sarkar, D (Mr Dipon Sarkar)
UTAS Author:Hunt, I (Dr Ian Hunt)
UTAS Author:Macdonald, CA (Mr Cameron Macdonald)
UTAS Author:Bowman, JP (Associate Professor John Bowman)
UTAS Author:Tamplin, ML (Professor Mark Tamplin)
ID Code:155424
Year Published:2023
Deposited By:TIA - Research Institute
Deposited On:2023-02-20
Last Modified:2023-02-20

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