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A novel R3H protein, OsDIP1, confers ABA‑mediated adaptation to drought and salinity stress in rice

Citation

Hang, L and Fu, WX and Ji, E and Tanveer, M and Shabala, S and Yu, M and Jiang, M, A novel R3H protein, OsDIP1, confers ABA‑mediated adaptation to drought and salinity stress in rice, Plant and soil, 477 pp. 501-519. ISSN 1573-5036 (2022) [Refereed Article]

Copyright Statement

The Author(s), under exclusive licence to Springer Nature Switzerland AG 2022

DOI: doi:10.1007/s11104-022-05428-y

Abstract

Aims Dehydration responsive element-binding factors (DBFs) have recently emerged as essential components of stress tolerance mechanisms in plants. In this work, we investigated the role of OsDIP1 that operate upstream of DBFs as a regulator of drought and salinity tolerance in rice.

Methods Experiments were conducted on rice plants (Oryza sativa) and included combination of physiological (plant phenotyping) and molecular methods. The latter involved real-time PCR (RT-qPCR); yeast two-hybrid, BiFC assay, GST pull-down, CoIP and firefly luciferase (LUC)/ renilla (REN) assays.

Results OsDIP1 expression was induced by hydrogen peroxide (H2O2), ABA (abscisic acid), drought (polyethylene glycol treatment), and salt stress. Overexpression of OsDIP1 in rice enhanced drought and salinity tolerance while knocking out OsDIP1 by CRISPR-Cas9 editing resulted in drought and salt sensitive phenotype. The activity and gene expression of antioxidant defense enzymes, superoxide dismutase (SOD), catalase (CAT), was increased in OsDIP1-overexpressed transgenic rice plants, while the content of malondialdehyde (MDA) was decreased. In contrast, the content and gene expression of SODCc2 and CatB, decreased, and the content of MDA was increased in knockout of OsDIP1 rice plants, suggesting that overexpression of OsDIP1 enhances the antioxidant capacity of rice plants. The yeast two-hybrid screening test revealed that OsDIP1 interacted with ZFP36, a key zinc finger transcription factor involved in ABA-induced antioxidant defense. Moreover, OsDIP1 could modulate some key ABA-responsive genes via interacting with ZFP36.

Conclusions In this work, we show that DIP1 plays a central role in modulating drought and salinity stress tolerance in rice.

Item Details

Item Type:Refereed Article
Keywords:ABA; drought; salinity
Research Division:Biological Sciences
Research Group:Plant biology
Research Field:Plant cell and molecular biology
Objective Division:Plant Production and Plant Primary Products
Objective Group:Other plant production and plant primary products
Objective Field:Climate adaptive plants
UTAS Author:Tanveer, M (Mr Mohsin Tanveer)
UTAS Author:Shabala, S (Professor Sergey Shabala)
ID Code:155152
Year Published:2022
Deposited By:Agriculture and Food Systems
Deposited On:2023-01-31
Last Modified:2023-03-22
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