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The mechanistic basis of sodium exclusion in Puccinellia tenuiflora under conditions of salinity and potassium deprivation
Citation
Han, Q-Q and Wang, Y-P and Li, J and Yin, X-C and Jiang, X-Y and Yu, M and Wang, S-M and Shabala, S and Zhang, J-L, The mechanistic basis of sodium exclusion in Puccinellia tenuiflora under conditions of salinity and potassium deprivation, The Plant journal : for cell and molecular biology, 112, (2) pp. 322-338. ISSN 1365-313X (2022) [Refereed Article]
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Abstract
Soil salinity is a significant threat to global agriculture. Understanding salt exclusion mechanisms in halophyte species may be instrumental in improving salt tolerance in crops. Puccinellia tenuiflora is a typical salt-excluding halophytic grass often found in potassium-deprived saline soils. Our previous work showed that P. tenuiflora possesses stronger selectivity for K+ than for Na+ ; however, the mechanistic basis of this phenomenon remained elusive. Here, P. tenuiflora PutHKT1;5 was cloned and the functions of PutHKT1;5 and PutSOS1 were characterized using heterologous expression systems. Yeast assays showed that PutHKT1;5 possessed Na+ transporting capacity and was highly selective for Na+ over K+ . PutSOS1 was located at the plasma membrane and operated as a Na+ /K+ exchanger, with much stronger Na+ extrusion capacity than its homolog from Arabidopsis. PutHKT2;1 mediated high-affinity K+ and Na+ uptake and its expression levels were upregulated by mild salinity and K+ deprivation. Salinity-induced changes of root PutHKT1;5 and PutHKT1;4 transcript levels matched the expression pattern of root PutSOS1, which was consistent with root Na+ efflux. The transcript levels of root PutHKT2;1 and PutAKT1 were downregulated by salinity. Taken together, these findings demonstrate that the functional activity of PutHKT1;5 and PutSOS1 in P. tenuiflora roots is fine-tuned under saline conditions as well as by operation of other ion transporters/channel (PutHKT1;4, PutHKT2;1, and PutAKT1). This leads to the coordination of radial Na+ and K+ transport processes, their loading to the xylem, or Na+ retrieval and extrusion under conditions of mild salinity and/or K+ deprivation.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Keywords: | salinity; sodium; potassium; Puccinellia |
| Research Division: | Biological Sciences |
| Research Group: | Plant biology |
| Research Field: | Plant physiology |
| Objective Division: | Plant Production and Plant Primary Products |
| Objective Group: | Other plant production and plant primary products |
| Objective Field: | Other plant production and plant primary products not elsewhere classified |
| UTAS Author: | Shabala, S (Professor Sergey Shabala) |
| ID Code: | 155138 |
| Year Published: | 2022 |
| Web of Science® Times Cited: | 2 |
| Deposited By: | Agriculture and Food Systems |
| Deposited On: | 2023-01-30 |
| Last Modified: | 2023-03-23 |
| Downloads: | 0 |
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