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Computational modeling and quantitative physiology reveal central parameters for brassinosteroid-regulated early cell physiological processes linked to elongation growth of the Arabidopsis root
Citation
Grobeholz, R and Wanke, F and Rohr, L and Glockner, N and Rausch, L and Scholl, S and Scacchi, E and Spazierer, A-J and Shabala, L and Shabala, S and Schumacher, K and Harter, K, Computational modeling and quantitative physiology reveal central parameters for brassinosteroid-regulated early cell physiological processes linked to elongation growth of the Arabidopsis root, eLife, 11 Article 7031. ISSN 2050-084X (2022) [Refereed Article]
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Abstract
Brassinosteroids (BR) are key hormonal regulators of plant development. However, whereas the individual components of BR perception and signaling are well characterized experimentally, the question of how they can act and whether they are sufficient to carry out the critical function of cellular elongation remains open. Here, we combined computational modeling with quantitative cell physiology to understand the dynamics of the plasma membrane (PM)-localized BR response pathway during the initiation of cellular responses in the epidermis of the Arabidopsis root tip that are be linked to cell elongation. The model, consisting of ordinary differential equations, comprises the BR-induced hyperpolarization of the PM, the acidification of the apoplast and subsequent cell wall swelling. We demonstrate that the competence of the root epidermal cells for the BR response predominantly depends on the amount and activity of H+-ATPases in the PM. The model further predicts that an influx of cations is required to compensate for the shift of positive charges caused by the apoplastic acidification. A potassium channel was subsequently identified and experimentally characterized, fulfilling this function. Thus, we established the landscape of components and parameters for physiological processes potentially linked to cell elongation, a central process in plant development.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Keywords: | brassinosteroids; hormonal regulation; growth; Arabidopsis |
| Research Division: | Biological Sciences |
| Research Group: | Plant biology |
| Research Field: | Plant cell and molecular biology |
| Objective Division: | Plant Production and Plant Primary Products |
| Objective Group: | Grains and seeds |
| Objective Field: | Grains and seeds not elsewhere classified |
| UTAS Author: | Shabala, L (Associate Professor Lana Shabala) |
| UTAS Author: | Shabala, S (Professor Sergey Shabala) |
| ID Code: | 155134 |
| Year Published: | 2022 |
| Deposited By: | Agriculture and Food Systems |
| Deposited On: | 2023-01-30 |
| Last Modified: | 2023-01-31 |
| Downloads: | 0 |
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