155132 - Stalk cell polar ion transport provide for bladder based salinity tolerance in.pdf (7.22 MB)
Stalk cell polar ion transport provide for bladder-based salinity tolerance in Chenopodium quinoa
journal contribution
posted on 2023-05-21, 16:05 authored by Bazihizina, N, Bohm, J, Messerer, M, Stigloher, C, Muller, HM, Tracey Cuin, Maierhofer, T, Cabot, J, Mayer, KFX, Fella, C, Huang, S, Al-Rasheid, KSA, Alquraishi, S, Michael BreadmoreMichael Breadmore, Mancuso, S, Sergey ShabalaSergey Shabala, Ache, P, Zhang, H, Zhu, J-K, Hendrich, R, Scherzer, SChenopodium quinoa uses epidermal bladder cells (EBCs) to sequester excess salt. Each EBC complex consists of a leaf epidermal cell, a stalk cell, and the bladder. Under salt stress, sodium (Na+ ), chloride (Cl- ), potassium (K+ ) and various metabolites are shuttled from the leaf lamina to the bladders. Stalk cells operate as both a selectivity filter and a flux controller. In line with the nature of a transfer cell, advanced transmission electron tomography, electrophysiology, and fluorescent tracer flux studies revealed the stalk cell's polar organization and bladder-directed solute flow. RNA sequencing and cluster analysis revealed the gene expression profiles of the stalk cells. Among the stalk cell enriched genes, ion channels and carriers as well as sugar transporters were most pronounced. Based on their electrophysiological fingerprint and thermodynamic considerations, a model for stalk cell transcellular transport was derived.
History
Publication title
The New phytologistVolume
235Issue
5Pagination
1822-1835ISSN
1469-8137Department/School
Tasmanian Institute of Agriculture (TIA)Publisher
Wiley on behalf of New Phytologist TrustPlace of publication
OxfordRights statement
Copyright 2022 The Authors. This is an open access article under the terms of the Creative Commons Attribution 4.0 International (CC BY 4.0) License, https://creativecommons.org/licenses/by/4.0/ which permits use, distribution and reproduction in any medium, provided the original work is properly cited.Repository Status
- Open