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155073 - Physiological and molecular responses of wheat low light intensity.pdf (3.89 MB)

Physiological and molecular responses of wheat to low light intensity

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posted on 2023-05-21, 16:00 authored by Li, X, Yang, R, Li, L, Ke LiuKe Liu, Matthew HarrisonMatthew Harrison, Fahad, S, Weil, M, Yin, L, Meixue ZhouMeixue Zhou, Wang, X
Here we document physiological and molecular attributes of three wheat cultivars (ZM9023, YM158 and FM1228) under low light intensity with advanced technologies, including non-standard quantitative technology and quantitative proteomics technology. We found lower dry matter accumulation of YM158 compared with ZM 9023 and FM1228 under low light intensities due to up-regulation of photosynthetic parameters electron transport rate (ETR), Y(II), Fv/Fm, Chl (a + b) of YM158 and down-regulation of Chl a/b. ETR, Y(II) and Fv/Fm significantly decreased between ZM9023 and FM1228. The ETR between PSII and PSI of YM158 increased, while light use efficiency (LUE) of ZM9023 and FM1228 decreased. We found that YM158 had greater propensity to adapt to low light compared with ZM9023, as the former was able to increase photochemical electron transfer rate, enhance photosystem activity, and increase the light energy under low light. This meant that the YM158 flag leaf has stronger regulatory mechanism under low light environment. Through proteomic analysis, we found LHC protein (LHCB1, LHCB4, LHCA2, LHCA3) for YH158 was significantly up-regulated, while the PSII subunit protein of FM1228 and ZM9023 b559 subunit protein were down-regulated. We also documented enhanced light use efficiency (LUE) due to higher light capture pigment protein complex (LHC), photosystem II (PSII), PSI and cytochrome B6F-related proteins, with dry matter accumulation being positively correlated with Fv/Fm, ETR, and ΦPS(II), and negatively correlated with initial fluorescence F0. We suggest that Fv/Fm, ETR, and ΦPS(II) could be considered in shade tolerance screening to facilitate wheat breeding.

Funding

Grains Research & Development Corporation

History

Publication title

Agronomy

Volume

13

Article number

272

Number

272

Pagination

1-22

ISSN

2073-4395

Department/School

Tasmanian Institute of Agriculture (TIA)

Publisher

MDPI AG

Place of publication

Switzerland

Rights statement

© 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution 4.0 International (CC BY 4.0) license, https://creativecommons.org/licenses/by/4.0/

Repository Status

  • Open

Socio-economic Objectives

Maize; Rice; Wheat