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Cathelicidin-3 associated with serum extracellular vesicles enables early diagnosis of a transmissible cancer
Citation
Espejo, C and Wilson, R and Pye, RJ and Ratcliffe, JC and Ruiz-Aravena, M and Willms, E and Wolfe, BW and Hamede, R and Hill, AF and Jones, ME and Woods, GM and Lyons, AB, Cathelicidin-3 associated with serum extracellular vesicles enables early diagnosis of a transmissible cancer, Frontiers in Immunology, 13 pp. 1-14. ISSN 1664-3224 (2022) [Refereed Article]
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DOI: doi:10.3389/fimmu.2022.858423
Abstract
The identification of practical early diagnostic biomarkers is a cornerstone of improved prevention and treatment of cancers. Such a case is devil facial tumor disease (DFTD), a highly lethal transmissible cancer afflicting virtually an entire species, the Tasmanian devil (Sarcophilus harrisii). Despite a latent period that can exceed one year, to date DFTD diagnosis requires visual identification of tumor lesions. To enable earlier diagnosis, which is essential for the implementation of effective conservation strategies, we analyzed the extracellular vesicle (EV) proteome of 87 Tasmanian devil serum samples using data-independent acquisition mass spectrometry approaches. The antimicrobial peptide cathelicidin-3 (CATH3), released by innate immune cells, was enriched in serum EV samples of both devils with clinical DFTD (87.9% sensitivity and 94.1% specificity) and devils with latent infection (i.e., collected while overtly healthy, but 3-6 months before subsequent DFTD diagnosis; 93.8% sensitivity and 94.1% specificity). Although high expression of antimicrobial peptides has been mostly related to inflammatory diseases, our results suggest that they can be also used as accurate cancer biomarkers, suggesting a mechanistic role in tumorous processes. This EV-based approach to biomarker discovery is directly applicable to improving understanding and diagnosis of a broad range of diseases in other species, and these findings directly enhance the capacity of conservation strategies to ensure the viability of the imperiled Tasmanian devil population.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Keywords: | exosomes, microvesicles, innate immunity, liquid biopsy, animal models, early cancer detection, proteomics, cathelicidins |
| Research Division: | Biomedical and Clinical Sciences |
| Research Group: | Immunology |
| Research Field: | Tumour immunology |
| Objective Division: | Expanding Knowledge |
| Objective Group: | Expanding knowledge |
| Objective Field: | Expanding knowledge in the biological sciences |
| UTAS Author: | Espejo, C (Mrs Camila Espejo) |
| UTAS Author: | Wilson, R (Dr Richard Wilson) |
| UTAS Author: | Pye, RJ (Ms Ruth Pye) |
| UTAS Author: | Ruiz-Aravena, M (Mr Manuel Ruiz Aravena) |
| UTAS Author: | Wolfe, BW (Mr Barrett Wolfe) |
| UTAS Author: | Hamede, R (Dr Rodrigo Hamede Ross) |
| UTAS Author: | Jones, ME (Mr Jones) |
| UTAS Author: | Woods, GM (Professor Gregory Woods) |
| UTAS Author: | Lyons, AB (Associate Professor Bruce Lyons) |
| ID Code: | 154957 |
| Year Published: | 2022 |
| Deposited By: | Menzies Institute for Medical Research |
| Deposited On: | 2023-01-20 |
| Last Modified: | 2023-01-20 |
| Downloads: | 0 |
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