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Seeing neurodegeneration in a new light using genetically encoded fluorescent biosensors and iPSCs

Citation

Stellon, D and Talbot, J and Hewitt, AW and King, AE and Cook, AL, Seeing neurodegeneration in a new light using genetically encoded fluorescent biosensors and iPSCs, International Journal of Molecular Sciences, 24, (2) pp. 1-26. ISSN 1422-0067 (2023) [Refereed Article]


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DOI: doi:10.3390/ijms24021766

Abstract

Neurodegenerative diseases present a progressive loss of neuronal structure and function, leading to cell death and irrecoverable brain atrophy. Most have disease-modifying therapies, in part because the mechanisms of neurodegeneration are yet to be defined, preventing the development of targeted therapies. To overcome this, there is a need for tools that enable a quantitative assessment of how cellular mechanisms and diverse environmental conditions contribute to disease. One such tool is genetically encodable fluorescent biosensors (GEFBs), engineered constructs encoding proteins with novel functions capable of sensing spatiotemporal changes in specific pathways, enzyme functions, or metabolite levels. GEFB technology therefore presents a plethora of unique sensing capabilities that, when coupled with induced pluripotent stem cells (iPSCs), present a powerful tool for exploring disease mechanisms and identifying novel therapeutics. In this review, we discuss different GEFBs relevant to neurodegenerative disease and how they can be used with iPSCs to illuminate unresolved questions about causes and risks for neurodegenerative disease.

Item Details

Item Type:Refereed Article
Keywords:stem cells, CRISPR/Cas technology, biosensor, neurodegeneration
Research Division:Biomedical and Clinical Sciences
Research Group:Neurosciences
Research Field:Cellular nervous system
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the biomedical and clinical sciences
UTAS Author:Stellon, D (Mr David Stellon)
UTAS Author:Talbot, J (Dr Jana Talbot)
UTAS Author:Hewitt, AW (Professor Alex Hewitt)
UTAS Author:King, AE (Professor Anna King)
UTAS Author:Cook, AL (Associate Professor Tony Cook)
ID Code:154870
Year Published:2023
Deposited By:Wicking Dementia Research and Education Centre
Deposited On:2023-01-17
Last Modified:2023-01-17
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