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Temporal changes in the microglial proteome of male and female mice after a diffuse brain injury using label-free quantitative proteomics


Doust, YV and Bindoff, A and Holloway, OG and Wilson, R and King, AE and Ziebell, JM, Temporal changes in the microglial proteome of male and female mice after a diffuse brain injury using label-free quantitative proteomics, Glia pp. 1-24. ISSN 0894-1491 (2022) [Refereed Article]

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DOI: doi:10.1002/glia.24313


Traumatic brain injury (TBI) triggers neuroinflammatory cascades mediated by microglia, which promotes tissue repair in the short-term. These cascades may exacerbate TBI-induced tissue damage and symptoms in the months to years post-injury. However, the progression of the microglial function across time post-injury and whether this differs between biological sexes is not well understood. In this study, we examined the microglial proteome at 3-, 7-, or 28-days after a midline fluid percussion injury (mFPI) in male and female mice using label-free quantitative proteomics. Data are available via ProteomeXchange with identifier PXD033628. We identified a reduction in microglial proteins involved with clearance of neuronal debris via phagocytosis at 3- and 7-days post-injury. At 28 days post-injury, pro-inflammatory proteins were decreased and anti-inflammatory proteins were increased in microglia. These results indicate a reduction in microglial clearance of neuronal debris in the days post-injury with a shift to anti-inflammatory function by 28 days following TBI. The changes in the microglial proteome that occurred across time post-injury did not differ between biological sexes. However, we did identify an increase in microglial proteins related to pro-inflammation and phagocytosis as well as insulin and estrogen signaling in males compared with female mice that occurred with or without a brain injury. Although the microglial response was similar between males and females up to 28 days following TBI, biological sex differences in the microglial proteome, regardless of TBI, has implications for the efficacy of treatment strategies targeting the microglial response post-injury.

Item Details

Item Type:Refereed Article
Keywords:biological sex, inflammation, microglia, proteomics, TBI, traumatic brain injury
Research Division:Biomedical and Clinical Sciences
Research Group:Neurosciences
Research Field:Cellular nervous system
Objective Division:Health
Objective Group:Clinical health
Objective Field:Diagnosis of human diseases and conditions
UTAS Author:Doust, YV (Miss Yasmine Doust)
UTAS Author:Bindoff, A (Mr Aidan Bindoff)
UTAS Author:Holloway, OG (Miss Olivia Holloway)
UTAS Author:Wilson, R (Dr Richard Wilson)
UTAS Author:King, AE (Professor Anna King)
UTAS Author:Ziebell, JM (Dr Jenna Ziebell)
ID Code:154833
Year Published:2022
Deposited By:Wicking Dementia Research and Education Centre
Deposited On:2023-01-11
Last Modified:2023-01-18

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