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Vascular perfusion differs in two distinct PDGFRβ-positive zones within the ischemic core of male mice 2 weeks following photothrombotic stroke

Citation

Morris, GP and Gowing, EK and Courtney, JM and Coombe, HE and King, NE and Rewell, SSJ and Howells, DW and Clarkson, AN and Sutherland, BA, Vascular perfusion differs in two distinct PDGFRβ-positive zones within the ischemic core of male mice 2 weeks following photothrombotic stroke, Vascular perfusion differs in two distinct PDGFRβ-positive zones within the ischemic core of male mice 2 weeks following photothrombotic stroke, Journal of neuroscience research, (101) Article 278-292. ISSN 1097-4547 (2023) [Refereed Article]


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Copyright Statement

Copyright (2023) The Authors. This is an open access article under the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/).

DOI: doi:10.1002/jnr.25146

Abstract

Stroke therapy has largely focused on preventing damage and encouraging repair outside the ischemic core, as the core is considered irreparable. Recently, several studies have suggested endogenous responses within the core are important for limiting the spread of damage and enhancing recovery, but the role of blood flow and capillary pericytes in this process is unknown. Using the Rose Bengal photothrombotic model of stroke, we illustrate blood vessels are present in the ischemic core and peri-lesional regions 2 weeks post stroke in male mice. A FITC-albumin gel cast of the vasculature revealed perfusion of these vessels, suggesting cerebral blood flow (CBF) may be partially present, without vascular leakage. The length of these vessels is significantly reduced compared to uninjured regions, but the average width is greater, suggesting they are either larger vessels that survived the initial injury, smaller vessels that have expanded in size (i.e., arteriogenesis), or that neovascularization begins with larger vessels. Concurrently, we observed an increase in platelet-derived growth factor receptor beta (PDGFRβ, a marker of pericytes) expression within the ischemic core in two distinct patterns, one which resembles pericyte-derived fibrotic scarring at the edge of the core, and one which is vessel associated and may represent blood vessel recovery. We find little evidence for dividing cells on these intralesional blood vessels 2 weeks post stroke. Our study provides evidence flow is present in PDGFRβ-positive vessels in the ischemic core 2 weeks post stroke. We hypothesize intralesional CBF is important for limiting injury and for encouraging endogenous repair following cerebral ischemia.

Item Details

Item Type:Refereed Article
Keywords:astrocyte, blood vessel, cell division, cerebral blood flow, core, ischemic stroke, perfusion, pericyte, peri-lesion
Research Division:Biomedical and Clinical Sciences
Research Group:Cardiovascular medicine and haematology
Research Field:Cardiology (incl. cardiovascular diseases)
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the biomedical and clinical sciences
UTAS Author:Morris, GP (Dr Gary Morris)
UTAS Author:Courtney, JM (Dr Jo-Maree Courtney)
UTAS Author:Coombe, HE (Ms Hannah Coombe)
UTAS Author:King, NE (Ms Natalie King)
UTAS Author:Howells, DW (Professor David Howells)
UTAS Author:Sutherland, BA (Associate Professor Brad Sutherland)
ID Code:154575
Year Published:2023
Funding Support:National Health and Medical Research Council (2003351)
Deposited By:Medicine
Deposited On:2022-12-13
Last Modified:2023-01-13
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