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Genome wide association study and haplotype analysis reveals the role of HvHKT1;5 in potassium retention but not Na+ exclusion in barley (Hordeum vulgare L.)

Citation

Zhu, Juan and Guo, Y and Zhou, H and Hong, H and Zhao, C and Shabala, SN and Choa, L and Guo, B and Zhou, Meixue and Xu, R, Genome wide association study and haplotype analysis reveals the role of HvHKT1;5 in potassium retention but not Na+ exclusion in barley (Hordeum vulgare L.), Environmental and Experimental Botany: An International Journal, 201 Article 104973. ISSN 0098-8472 (2022) [Refereed Article]

Copyright Statement

2022 Elsevier B.V. All rights reserved.

DOI: doi:10.1016/j.envexpbot.2022.104973

Abstract

Salinity stress is one of the major abiotic stresses which affect grain yield and quality in crops. Identifying and exploring the QTL/genes and elite alleles that control salt tolerance is crucial for breeding salt-tolerant crop varieties. In this study, a diverse panel of 214 barley (Hordeum vulgare L.) accessions were used to identify quantitative trait loci (QTL) for salt tolerance and Na+ and K+ content in leaves under salt stress by MLM model and MapQTL6.0 software. Significant correlations between leaf ion content and salt tolerance were observed. One major QTL, Q.SL.2H, was identified on chromosome 2H and was responsible for the overall salinity tolerance through regulating Na+ exclusion under salt stress. The other major QTL was detected on chromosome 4H that overlapped with HvHKT1;5 gene and was responsible for K+ retention in leaves. Several other minor QTL were detected by MapQTL that could help in selecting salt-tolerant varieties. The haplotype analysis showed that amino acid substitutions of HvHKT1;5 in exon2 at 416 and 438 may have contributed to K+ retention under salinity stress. These findings indicate the existence of two major mechanisms for salinity tolerance determined by two major QTL and challenge the accepted views on the role of HvHKT1;5 transporters in Na+ exclusion in barley adaptive response to salinity stress.

Item Details

Item Type:Refereed Article
Keywords:barley salinity
Research Division:Agricultural, Veterinary and Food Sciences
Research Group:Crop and pasture production
Research Field:Agrochemicals and biocides (incl. application)
Objective Division:Plant Production and Plant Primary Products
Objective Group:Grains and seeds
Objective Field:Wheat
UTAS Author:Zhu, Juan (Miss Juan Zhu)
UTAS Author:Zhao, C (Dr Chenchen Zhao)
UTAS Author:Shabala, SN (Professor Sergey Shabala)
UTAS Author:Zhou, Meixue (Professor Meixue Zhou)
ID Code:154338
Year Published:2022
Deposited By:TIA - Research Institute
Deposited On:2022-11-23
Last Modified:2022-12-13
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