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Comparative proteomic analysis of potato roots from resistant and susceptible cultivars to Spongospora subterranea zoospore root attachment in vitro

Citation

Yu, X and Wilson, R and Balotf, S and Tegg, RS and Eyles, A and Wilson, CR, Comparative proteomic analysis of potato roots from resistant and susceptible cultivars to Spongospora subterranea zoospore root attachment in vitro, Molecules: A Journal of Synthetic Organic and Natural Product Chemistry, 27, (18) Article 6024. ISSN 1420-3049 (2022) [Refereed Article]


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Copyright Statement

2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution 4.0 International (CC BY 4.0) license (https://creativecommons.org/license s/by/4.0/).

DOI: doi:10.3390/molecules27186024

Abstract

Potato (Solanum tuberosum L.) exhibits broad variations in cultivar resistance to tuber and root infections by the soilborne, obligate biotrophic pathogen Spongospora subterranea. Host resistance has been recognised as an important approach in potato disease management, whereas zoospore root attachment has been identified as an effective indicator for the host resistance to Spongospora root infection. However, the mechanism of host resistance to zoospore root attachment is currently not well understood. To identify the potential basis for host resistance to S. subterranea at the molecular level, twelve potato cultivars differing in host resistance to zoospore root attachment were used for comparative proteomic analysis. In total, 3723 proteins were quantified from root samples across the twelve cultivars using a data-independent acquisition mass spectrometry approach. Statistical analysis identified 454 proteins that were significantly more abundant in the resistant cultivars; 626 proteins were more abundant in the susceptible cultivars. In resistant cultivars, functional annotation of the proteomic data indicated that Gene Ontology terms related to the oxidative stress and metabolic processes were significantly over-represented. KEGG pathway analysis identified that the phenylpropanoid biosynthesis pathway was associated with the resistant cultivars, suggesting the potential role of lignin biosynthesis in the host resistance to S. subterranea. Several enzymes involved in pectin biosynthesis and remodelling, such as pectinesterase and pectin acetylesterase, were more abundant in the resistant cultivars. Further investigation of the potential role of root cell wall pectin revealed that the pectinase treatment of roots resulted in a significant reduction in zoospore root attachment in both resistant and susceptible cultivars. This study provides a comprehensive proteome-level overview of resistance to S. subterranea zoospore root attachment across twelve potato cultivars and has identified a potential role for cell wall pectin in regulating zoospore root attachment.

Item Details

Item Type:Refereed Article
Keywords:proteomics, spongospora, potato, Spongospora subterranea, Solanum tuberosum, label-free proteomics, DIA, zoospore root attachment, host resistance
Research Division:Agricultural, Veterinary and Food Sciences
Research Group:Crop and pasture production
Research Field:Crop and pasture protection (incl. pests, diseases and weeds)
Objective Division:Plant Production and Plant Primary Products
Objective Group:Horticultural crops
Objective Field:Field grown vegetable crops
UTAS Author:Yu, X (Ms Xian Yu)
UTAS Author:Wilson, R (Dr Richard Wilson)
UTAS Author:Balotf, S (Mr Sadegh Balotf)
UTAS Author:Tegg, RS (Dr Robert Tegg)
UTAS Author:Eyles, A (Dr Alieta Eyles)
UTAS Author:Wilson, CR (Professor Calum Wilson)
ID Code:154063
Year Published:2022
Deposited By:TIA - Research Institute
Deposited On:2022-10-26
Last Modified:2022-12-19
Downloads:0

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