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Development and validation of a novel rapid in vitro assay for determining resistance of potato cultivars to root attachment by Spongospora subterranea zoospores

Citation

Yu, X and Tegg, RS and Eyles, A and Wilson, AJ and Wilson, CR, Development and validation of a novel rapid in vitro assay for determining resistance of potato cultivars to root attachment by Spongospora subterranea zoospores, Plant Pathology, 72, (2) pp. 392-405. ISSN 1365-3059 (2022) [Refereed Article]


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Copyright Statement

2022 The Authors. Plant Pathology published by John Wiley & Sons Ltd on behalf of British Society for Plant Pathology. This is an open access article under the terms of the Creative Commons Attribution 4.0 International (CC BY 4.0) License. https://creativecommons.org/licenses/by/4.0/

DOI: doi:10.1111/ppa.13659

Abstract

Spongospora subterranea f. sp. subterranea is a major pathogen of potatoes leading to losses in tuber quality and yield. Disease can be expressed as root infection, root galling and tuber lesions, the latter known as powdery scab. Attachment of zoospores to potato root hairs is the first step before infection of roots and disease development. Root hair infection results in root dysfunction leading to impaired plant productivity and yield. Varieties vary in their susceptibility to root and tuber disease; however, varietal screening is both time and resource intensive. Furthermore, traditional screens assess root galling or tuber disease and not root infection. In this study, we determined optimal conditions for zoospore release and attachment of zoospores to plant roots and used this information to develop an in vitro bioassay to assess resistance to zoospore root attachment among 153 potato lines and cultivars. Optimal zoospore release occurred at 20C in Hoagland's solution in a rapid and synchronized manner over the first 2 days, followed by a steep decline. The extent of zoospore root attachment varied with cultivar (Iwa > Agria > Russet Burbank > Gladiator), region of the root maturation zone (lower > middle > upper) and temperature (greatest zoospore root attachment occurring at 15C). Further comparisons suggested efficiency of zoospore root attachment was also generally associated with known variety resistance to powdery scab, zoosporangial infection and root galling, with a few notable exceptions. The bioassay proved to be a rapid and robust method for screening cultivar resistance to zoospore root attachment.

Item Details

Item Type:Refereed Article
Keywords:in vitro assay, Spongospora subterreanea, host resistance, in vitro assay, root infection, Spongospora subterranea f. sp. subterranea, zoospore germination, zoospore root attachment
Research Division:Agricultural, Veterinary and Food Sciences
Research Group:Crop and pasture production
Research Field:Crop and pasture protection (incl. pests, diseases and weeds)
Objective Division:Plant Production and Plant Primary Products
Objective Group:Horticultural crops
Objective Field:Field grown vegetable crops
UTAS Author:Yu, X (Ms Xian Yu)
UTAS Author:Tegg, RS (Dr Robert Tegg)
UTAS Author:Eyles, A (Dr Alieta Eyles)
UTAS Author:Wilson, AJ (Mrs Annabel Wilson)
UTAS Author:Wilson, CR (Professor Calum Wilson)
ID Code:154061
Year Published:2022
Deposited By:TIA - Research Institute
Deposited On:2022-10-26
Last Modified:2023-01-11
Downloads:1 View Download Statistics

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