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Sarcoptic mange changes bacterial and fungal microbiota of bare-nosed wombats (Vombatus ursinus)

Citation

Naesborg-Nielsen, C and Eisenhofer, R and Fraser, TA and Wilkinson, V and Burridge, CP and Carver, S, Sarcoptic mange changes bacterial and fungal microbiota of bare-nosed wombats (Vombatus ursinus), Parasites & Vectors, 15 Article 323. ISSN 1756-3305 (2022) [Refereed Article]


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Copyright Statement

2022. The Authors. This article is licensed under a Creative Commons Attribution 4.0 International (CC BY 4.0) License (https://creativecommons.org/licenses/by/4.0/), which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

DOI: doi:10.1186/s13071-022-05452-y

Abstract

Background

Sarcoptes scabiei is globally distributed and one of the most impactful mammalian ectoparasites. Sarcoptic mange, caused by infection with S. scabiei, causes disruption of the epidermis and its bacterial microbiota, but its effects on host fungal microbiota and on the microbiota of marsupials in general have not been studied. Here, we (i) examine bacterial and fungal microbiota changes associated with mange in wild bare-nosed wombats (BNWs) and (ii) evaluate whether opportunistic pathogens are potentiated by S. scabiei infection in this species.

Methods

Using Amplicon Sequencing of the 16S rRNA and ITS2 rDNA genes, we detected skin microbiota changes of the bare-nosed wombat (Vombatus ursinus). We compared the alpha and beta diversity among healthy, moderate, and severe disease states using ANOVA and PERMANOVA with nesting. Lastly, we identified taxa that differed between disease states using analysis of composition of microbes (ANCOM) testing.

Results

We detected significant changes in the microbial communities and diversity with mange in BNWs. Severely affected BNWs had lower amplicon sequence variant (ASV) richness compared to that of healthy individuals, and the microbial communities were significantly different between disease states with higher relative abundance of potentially pathogenic microbial taxa in mange-affected BNWs including Staphylococcus sciuri, Corynebacterium spp., Brevibacterium spp., Brachybacterium spp., and Pseudogymnascus spp. and Debaryomyces spp.

Conclusion

This study represents the first investigation of microbial changes in association with sarcoptic mange in a marsupial host, as well as the first investigation of fungal microbial changes on the skin of any host suffering from sarcoptic mange. Our results are broadly consistent with bacterial microbiota changes observed in humans, pigs, canids, and Iberian ibex, suggesting the epidermal microbial impacts of mange may be generalisable across host species. We recommend that future studies investigating skin microbiota changes include both bacterial and fungal data to gain a more complete picture of the effects of sarcoptic mange.

Item Details

Item Type:Refereed Article
Keywords:wombat, sarcoptic mange, Sarcoptes scabiei, bare-nosed wombat, microbiota, dysbiosis, Staphylococcus sciuri, corynebacterium, pseudogymnascus, debaryomyces
Research Division:Biological Sciences
Research Group:Ecology
Research Field:Terrestrial ecology
Objective Division:Environmental Management
Objective Group:Terrestrial systems and management
Objective Field:Control of pests, diseases and exotic species in terrestrial environments
UTAS Author:Naesborg-Nielsen, C (Ms Christina Naesborg-Nielsen)
UTAS Author:Fraser, TA (Ms Tamieka Fraser)
UTAS Author:Wilkinson, V (Miss Victoria Wilkinson)
UTAS Author:Burridge, CP (Associate Professor Christopher Burridge)
UTAS Author:Carver, S (Associate Professor Scott Carver)
ID Code:154059
Year Published:2022
Deposited By:Zoology
Deposited On:2022-10-26
Last Modified:2022-12-02
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