eCite Digital Repository

Characterization and genomic analysis of ssDNA vibriophage vB_VpaM_PG19 within Microviridae, representing a novel viral genus

Citation

Guo, R and Zheng, K and Luo, L and Liu, Y and Shao, H and Guo, C and He, H and Wang, H and Sung, YY and Mok, WJ and Wong, LL and Zhang, Y-Z and Liang, Y and McMinn, A and Wang, M, Characterization and genomic analysis of ssDNA vibriophage vB_VpaM_PG19 within Microviridae, representing a novel viral genus, Microbiology Spectrum, 10, (4) Article e00585-22. ISSN 2165-0497 (2022) [Refereed Article]


Preview
PDF (Published version)
3Mb
  

Copyright Statement

2022 Guo et al. This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International (CC BY 4.0) License, (https://creativecommons.org/licenses/by/4.0/)

DOI: doi:10.1128/spectrum.00585-22

Abstract

Vibrio parahaemolyticus, a widespread marine bacterium, is responsible for a variety of diseases in marine organisms. Consumption of raw or undercooked seafood contaminated with V. parahaemolyticus is also known to cause acute gastroenteritis in humans. While numerous dsDNA vibriophages have been isolated so far, there have been few studies of vibriophages belonging to the ssDNA Microviridae family. In this study, a novel ssDNA phage, vB_VpaM_PG19 infecting V. parahaemolyticus, with a 5,572 bp ssDNA genome with a G+C content of 41.31% and encoded eight open reading frames, was isolated. Genome-wide phylogenetic analysis of the total phage isolates in the GenBank database revealed that vB_VpaM_PG19 was only related to the recently deposited vibriophage vB_VpP_WS1. The genome-wide average nucleotide homology of the two phages was 89.67%. The phylogenetic tree and network analysis showed that vB_VpaM_PG19 was different from other members of the Microviridae family and might represent a novel viral genus, together with vibriophage vB_VpP_WS1, named Vimicrovirus. One-step growth curves showed that vB_VpaM_PG19 has a short incubation period, suggesting its potential as an antimicrobial agent for pathogenic V. parahaemolyticus.

Item Details

Item Type:Refereed Article
Keywords:Vibrio, phage vB_VpaM_PG19, Microviridae, genomic and phylogenetic analysis
Research Division:Biological Sciences
Research Group:Microbiology
Research Field:Microbial ecology
Objective Division:Environmental Management
Objective Group:Coastal and estuarine systems and management
Objective Field:Coastal or estuarine biodiversity
UTAS Author:McMinn, A (Professor Andrew McMinn)
ID Code:153648
Year Published:2022
Deposited By:Ecology and Biodiversity
Deposited On:2022-09-29
Last Modified:2022-11-28
Downloads:3 View Download Statistics

Repository Staff Only: item control page