153648 - Characterization and genomic analysis of ssDNA vibriophage.pdf (3.15 MB)
Characterization and genomic analysis of ssDNA vibriophage vB_VpaM_PG19 within Microviridae, representing a novel viral genus
journal contribution
posted on 2023-05-21, 13:59 authored by Guo, R, Zheng, K, Luo, L, Liu, Y, Shao, H, Guo, C, He, H, Wang, H, Sung, YY, Mok, WJ, Wong, LL, Zhang, Y-Z, Liang, Y, Andrew McMinnAndrew McMinn, Wang, MVibrio parahaemolyticus, a widespread marine bacterium, is responsible for a variety of diseases in marine organisms. Consumption of raw or undercooked seafood contaminated with V. parahaemolyticus is also known to cause acute gastroenteritis in humans. While numerous dsDNA vibriophages have been isolated so far, there have been few studies of vibriophages belonging to the ssDNA Microviridae family. In this study, a novel ssDNA phage, vB_VpaM_PG19 infecting V. parahaemolyticus, with a 5,572 bp ssDNA genome with a G+C content of 41.31% and encoded eight open reading frames, was isolated. Genome-wide phylogenetic analysis of the total phage isolates in the GenBank database revealed that vB_VpaM_PG19 was only related to the recently deposited vibriophage vB_VpP_WS1. The genome-wide average nucleotide homology of the two phages was 89.67%. The phylogenetic tree and network analysis showed that vB_VpaM_PG19 was different from other members of the Microviridae family and might represent a novel viral genus, together with vibriophage vB_VpP_WS1, named Vimicrovirus. One-step growth curves showed that vB_VpaM_PG19 has a short incubation period, suggesting its potential as an antimicrobial agent for pathogenic V. parahaemolyticus.
History
Publication title
Microbiology SpectrumVolume
10Issue
4Article number
e00585-22Number
e00585-22Pagination
1-12ISSN
2165-0497Department/School
Institute for Marine and Antarctic StudiesPublisher
American Society for MicrobiologyPlace of publication
United StatesRights statement
© 2022 Guo et al. This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International (CC BY 4.0) License, (https://creativecommons.org/licenses/by/4.0/)Repository Status
- Open