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Root K+ homeostasis and signalling as a determinant of salinity stress tolerance in cultivated and wild rice species

Citation

Shahzad, B and Yun, P and Rasouli, F and Shabala, SI and Zhou, Meixue and Venkataraman, G and Chen, ZH and Shabala, SN, Root K+ homeostasis and signalling as a determinant of salinity stress tolerance in cultivated and wild rice species, Environmental and Experimental Botany, 201 Article 104944. ISSN 0098-8472 (2022) [Refereed Article]

Copyright Statement

2022 Elsevier B.V. All rights reserved

DOI: doi:10.1016/j.envexpbot.2022.104944

Abstract

Cytosolic K+ retention is an important determinant of salinity tolerance in many crops. However, it remains unclear whether this trait is also crucial for wild rice species. In this work, contrasting pairs of cultivated (Oryza sativa L.) and wild (Oryza alta; Oryza punctata) rice species were used to understand the mechanistic basis of salinity stress tolerance in rice and the role of K+ retention in this process. Non-invasive Microelectrode Ion Flux Measuring (MIFE) measurements showed that NaCl and ROS-induced K+ efflux from the elongation root zone were significantly (2-3-fold) higher in the wild rice species compared with their cultivated counterparts. Cultivated rice group showed relatively lower K+ efflux but greater H+ efflux in response to NaCl and ROS treatments. Pharmacological experiments revealed that tetraethylammonium chloride (TEA) and gadolinium chloride (GdCl3) markedly suppressed NaCl and ROS-induced K+ efflux (>80 % inhibition), suggesting the involvement of GORK and NSCC channels in stress-induced K+ efflux. Sodium orthovanadate suppressed H+ efflux (>90 % inhibition), suggesting the role of H+-ATPase as a major source in salt-induced H+ efflux in salt tolerant cultivars. Collectively, our results indicate that, while possessing high root K+ retention ability in the mature zone, wild rice species show higher sensitivity to NaCl and ROS in the elongation zone. It is suggested that stress-induced K+ efflux in this zone may operate as a "metabolic switch" by inhibiting energy consuming anabolic reactions and allowing energy to be saved for adaptations and repairs, which may provide an advantage to wild rice in conditions with high salinity.

Item Details

Item Type:Refereed Article
Keywords:rice; salinity
Research Division:Agricultural, Veterinary and Food Sciences
Research Group:Crop and pasture production
Research Field:Agrochemicals and biocides (incl. application)
Objective Division:Plant Production and Plant Primary Products
Objective Group:Grains and seeds
Objective Field:Rice
UTAS Author:Shahzad, B (Mr Babar Shahzad)
UTAS Author:Yun, P (Mr Ping Yun)
UTAS Author:Shabala, SI (Associate Professor Lana Shabala)
UTAS Author:Zhou, Meixue (Professor Meixue Zhou)
UTAS Author:Shabala, SN (Professor Sergey Shabala)
ID Code:153048
Year Published:2022
Web of Science® Times Cited:2
Deposited By:TIA - Research Institute
Deposited On:2022-09-02
Last Modified:2022-11-18
Downloads:0

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