eCite Digital Repository
A self-labeling protein based on the small ultra-red fluorescent protein, smURFP
Citation
Machado, JH and Ting, R and Lin, JYL and Rodriguez, EA, A self-labeling protein based on the small ultra-red fluorescent protein, smURFP, RSC Chemical Biology, 2, (4) pp. 1221-1226. ISSN 2633-0679 (2021) [Refereed Article]
 | PDF 3Mb |
Copyright Statement
Copyright 2021 The Authors Licensed under Creative Commons Attribution-NonCommercial 3.0 Unported (CC BY-NC 3.0) https://creativecommons.org/licenses/by-nc/3.0/
Abstract
Self-labeling proteins have revolutionized super-resolution and sensor imaging. Tags recognize a bioorthogonal substrate for covalent attachment. We show the small Ultra-Red Fluorescent Protein (smURFP) is a self-labeling protein. The substrate is fluorogenic, fluoresces when attached, and quenches fluorescent cargo. The smURFP-tag has novel properties for tool development.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Keywords: | unnatural amino acids, site specific incorporation, suppessor transer RNAs, IX beta reductase, general method, in vivo, improved amber, cell, technology, molecules |
| Research Division: | Chemical Sciences |
| Research Group: | Medicinal and biomolecular chemistry |
| Research Field: | Biomolecular modelling and design |
| Objective Division: | Expanding Knowledge |
| Objective Group: | Expanding knowledge |
| Objective Field: | Expanding knowledge in the biological sciences |
| UTAS Author: | Lin, JYL (Dr John Lin) |
| ID Code: | 152854 |
| Year Published: | 2021 |
| Web of Science® Times Cited: | 1 |
| Deposited By: | Medicine |
| Deposited On: | 2022-08-25 |
| Last Modified: | 2022-10-28 |
| Downloads: | 1 View Download Statistics |
Repository Staff Only: item control page