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A self-labeling protein based on the small ultra-red fluorescent protein, smURFP


Machado, JH and Ting, R and Lin, JYL and Rodriguez, EA, A self-labeling protein based on the small ultra-red fluorescent protein, smURFP, RSC Chemical Biology, 2, (4) pp. 1221-1226. ISSN 2633-0679 (2021) [Refereed Article]


Copyright Statement

Copyright 2021 The Authors Licensed under Creative Commons Attribution-NonCommercial 3.0 Unported (CC BY-NC 3.0)

DOI: doi:10.1039/d1cb00127b


Self-labeling proteins have revolutionized super-resolution and sensor imaging. Tags recognize a bioorthogonal substrate for covalent attachment. We show the small Ultra-Red Fluorescent Protein (smURFP) is a self-labeling protein. The substrate is fluorogenic, fluoresces when attached, and quenches fluorescent cargo. The smURFP-tag has novel properties for tool development.

Item Details

Item Type:Refereed Article
Keywords:unnatural amino acids, site specific incorporation, suppessor transer RNAs, IX beta reductase, general method, in vivo, improved amber, cell, technology, molecules
Research Division:Chemical Sciences
Research Group:Medicinal and biomolecular chemistry
Research Field:Biomolecular modelling and design
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the biological sciences
UTAS Author:Lin, JYL (Dr John Lin)
ID Code:152854
Year Published:2021
Web of Science® Times Cited:1
Deposited By:Medicine
Deposited On:2022-08-25
Last Modified:2022-10-28
Downloads:1 View Download Statistics

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