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Microsensors in plant biology: In vivo visualization of inorganic analytes with high spatial and/or temporal resolution

Citation

Pedersen, O and Revsbech, NP and Shabala, SN, Microsensors in plant biology: In vivo visualization of inorganic analytes with high spatial and/or temporal resolution, Journal of Experimental Botany, 71, (14) pp. 3941-3954. ISSN 0022-0957 (2020) [Refereed Article]


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DOI: doi:10.1093/jxb/eraa175

Abstract

This Expert View provides an update on the recent development of new microsensors, and briefly summarizes some novel applications of existing microsensors, in plant biology research. Two major topics are covered: (i) sensors for gaseous analytes (O2, CO2, and H2S); and (ii) those for measuring concentrations and fluxes of ions (macro- and micronutrients and environmental pollutants such as heavy metals). We show that application of such microsensors may significantly advance understanding of mechanisms of plant-environmental interaction and regulation of plant developmental and adaptive responses under adverse environmental conditions via non-destructive visualization of key analytes with high spatial and/or temporal resolution. Examples included cover a broad range of environmental situations including hypoxia, salinity, and heavy metal toxicity. We highlight the power of combining microsensor technology with other advanced biophysical (patch-clamp, voltage-clamp, and single-cell pressure probe), imaging (MRI and fluorescent dyes), and genetic techniques and approaches. We conclude that future progress in the field may be achieved by applying existing microsensors for important signalling molecules such as NO and H2O2, by improving selectivity of existing microsensors for some key analytes (e.g. Na, Mg, and Zn), and by developing new microsensors for P.

Item Details

Item Type:Refereed Article
Keywords:ion flux, MIFE, micro-optode, microelectrode, tissue CO2, tissue O2
Research Division:Biological Sciences
Research Group:Plant biology
Research Field:Plant biochemistry
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the environmental sciences
UTAS Author:Shabala, SN (Professor Sergey Shabala)
ID Code:152759
Year Published:2020
Web of Science® Times Cited:10
Deposited By:Research Division
Deposited On:2022-08-24
Last Modified:2022-08-24
Downloads:0

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