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Microsensors in plant biology: In vivo visualization of inorganic analytes with high spatial and/or temporal resolution
journal contribution
posted on 2023-05-21, 12:46 authored by Pedersen, O, Revsbech, NP, Sergey ShabalaSergey ShabalaThis Expert View provides an update on the recent development of new microsensors, and briefly summarizes some novel applications of existing microsensors, in plant biology research. Two major topics are covered: (i) sensors for gaseous analytes (O2, CO2, and H2S); and (ii) those for measuring concentrations and fluxes of ions (macro- and micronutrients and environmental pollutants such as heavy metals). We show that application of such microsensors may significantly advance understanding of mechanisms of plant-environmental interaction and regulation of plant developmental and adaptive responses under adverse environmental conditions via non-destructive visualization of key analytes with high spatial and/or temporal resolution. Examples included cover a broad range of environmental situations including hypoxia, salinity, and heavy metal toxicity. We highlight the power of combining microsensor technology with other advanced biophysical (patch-clamp, voltage-clamp, and single-cell pressure probe), imaging (MRI and fluorescent dyes), and genetic techniques and approaches. We conclude that future progress in the field may be achieved by applying existing microsensors for important signalling molecules such as NO and H2O2, by improving selectivity of existing microsensors for some key analytes (e.g. Na, Mg, and Zn), and by developing new microsensors for P.
History
Publication title
Journal of Experimental BotanyVolume
71Issue
14Pagination
3941-3954ISSN
0022-0957Department/School
Tasmanian Institute of Agriculture (TIA)Publisher
Oxford Univ PressPlace of publication
Great Clarendon St, Oxford, England, Ox2 6DpRights statement
© 2020 The Author(s).Repository Status
- Restricted