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Cyclodextrins as mobile phase additives in open-tubular admicellar electrochromatography for achiral and chiral separations


Yu, RB and Quirino, JP, Cyclodextrins as mobile phase additives in open-tubular admicellar electrochromatography for achiral and chiral separations, Microchemical Journal, 161 Article 105763. ISSN 0026-265X (2021) [Refereed Article]

DOI: doi:10.1016/j.microc.2020.105763


Native cyclodextrins (alpha-, beta- and gamma-CD) which are traditional chiral selectors in capillary electrophoresis were studied in open-tubular admicellar electrochromatography (OT-AMEC) using cetyltrimethylammonium bromide (CTAB) as dynamic stationary pseudophase. The initial mobile phase was 0.2 mM CTAB in 25 mM sodium tetraborate (pH 9.2), which forms admicelles (bilayer) at the solid surface/liquid interface inside the capillary. Different molar ratios of [CD]:[CTAB], and 0:1 to 2:1 for [alpha-CD]:[CTAB] and [beta-CD]:[CTAB] and 0:1 to 20:1 for [gamma-CD]:[CTAB] in the mobile phase were investigated. An increase in the CD concentration decreased the magnitude of the electrosmotic flow (EOF). This suggests that CDs increase the critical surface aggregation concentration of CTAB, reducing the amount of free CTAB available to aggregate at the solid surface/liquid interface. At a molar ratio of 1.5:1 for alpha-CD, 2:1 for beta-CD and 20:1 for gamma-CD, the EOF changed from anodic to cathodic in direction. This suggests that no admicelles were formed under these conditions. Consequently, the retention factors (k) of the tested analytes (dichlorprop, fenoprop, ibuprofen, ketoprofen, propranolol, verapamil, hexanophenone, verapamil and valerophenone) decreased until k = 0 (no admicelles or stationary pseudophase). We observed a change in the migration orders of the analytes because of the resulting change in the effective electrophoretic mobility. Finally, we achieved chiral separation of mecoprop and dichlorprop in OT-AMEC with alpha- or beta-CD in the mobile phase, which was not possible in electrokinetic chromatography using the same alpha- or beta-CD concentration in the background solution. The chiral separation was aided by the retention caused by the dynamic stationary pseudophase.

Item Details

Item Type:Refereed Article
Keywords:Capillary electrochromatography, Chiral separation, Cetyltrimethy lammonium bromide,Cyclodextrins,Open-tubular,Selectivity modification
Research Division:Chemical Sciences
Research Group:Analytical chemistry
Research Field:Separation science
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the biomedical and clinical sciences
UTAS Author:Quirino, JP (Associate Professor Lito Quirino)
ID Code:152688
Year Published:2021
Web of Science® Times Cited:7
Deposited By:Medicine
Deposited On:2022-08-23
Last Modified:2022-09-12

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