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Male specific DNA sequences were selected from a Diversity Arrays Technology (DArT) mapping study to evaluate their suitability for determination of the sex phenotype among young seedlings in a hop (Humulus lupulus L.) breeding program. Ten male specific DArT markers showed complete linkage with male sex phenotype in three crossing families. Following optimization, four were successfully converted into PCR markers and a multiplex PCR approach for their use was developed. Among 197 plants (97 from the world collection; 100 from three segregating families), 94-100% positive correlation with sex phenotypic data was achieved for the single PCR amplification, whereas the multiplex approach showed 100% correlation. To develop a fast and low-cost method, crude sample multiplex PCR was evaluated in 253 progenies from 14 segregating populations without losing accuracy. The study describes, for the first time, the routine application of molecular markers linked to male sex in an intensive Slovenian hop breeding program. The methods described could be employed for screening of sex at the seedling stage in other hop programs worldwide, thereby saving resources for desirable female plants.

Item Type:	Refereed Article
Keywords:	Diversity Arrays Technology, genetic mapping, Humulus lupulus, hops, hop breeding
Research Division:	Agricultural, Veterinary and Food Sciences
Research Group:	Horticultural production
Research Field:	Horticultural crop improvement (incl. selection and breeding)
Objective Division:	Plant Production and Plant Primary Products
Objective Group:	Industrial crops
Objective Field:	Hops
UTAS Author:	Whittock, SP (Mr Simon Whittock)
UTAS Author:	Koutoulis, A (Professor Anthony Koutoulis)
ID Code:	152608
Year Published:	2019
Web of Science® Times Cited:	7
Deposited By:	Research Performance and Analysis
Deposited On:	2022-08-22
Last Modified:	2022-09-20
Downloads:	5 View Download Statistics