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Paperfluidic devices with a selective molecularly imprinted polymer surface for instrumentation-free distance-based detection of protein biomarkers


Rypar, T and Adam, V and Vaculovicova, M and Macka, M, Paperfluidic devices with a selective molecularly imprinted polymer surface for instrumentation-free distance-based detection of protein biomarkers, Sensors and Actuators B: Chemical, 341 Article 129999. ISSN 0925-4005 (2021) [Refereed Article]

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DOI: doi:10.1016/j.snb.2021.129999


Microfluidic paper-based analytical devices (mu PADs) offer the advantages of simplicity, extremely low costs, robustness, and miniaturisation, synergistically supporting portability and point-of-care (POC) analysis. When mu PADs are combined with distance-based detection in D mu PADs, they uniquely enable a quantitative analytical platform that is truly instrumentation-free (naked-eye readout), or at least, does not require any specialised scientific instrumentation (only mobile phone camera). However, a significant drawback of D mu PADs is their limited selectivity. In this work, we present for the first time molecularly imprinted polymer (MIP) as a selectivity-enhancing element in MIP-modified D mu PADs (MIP-D mu PADs). Herein, a layer of polydopamine MIP was coated onto the paper substrate of a D mu PAD, in a simple process using dopamine as the monomer deposited onto the paper matrix in the migration-detection zone of the D mu PAD, and polymerised in a rapid low cost procedure in the presence of oxygen under alkaline conditions. The polydopamine MIP-D mu PAD was then systematically investigated for the selective determination of chymotrypsinogen (chymo) as a model protein biomarker in urine, within the linear concentration range 2.4-29.2 mu M (R2 = 0,9903) with corresponding relative standard deviations ranging from 2% to 11 % and LOD =3.5 mu M and LOQ =11.8 mu M. The here presented analytical concept based on MIP-D mu PADs has a potential in POC diagnostics, because of the combination of low cost automated fabrication, the rapid quantitative near to instrumentation-free analysis, and selectivity through the use of MIPs as a synthetic, more stable, cheaper and easily prepared alternative to bio-macromolecules.

Item Details

Item Type:Refereed Article
Keywords:paperfluidic devices, paper-based analysis, distance-based detection, molecularly imprinted polymer, polydopamine MIP, protein biomarkers
Research Division:Chemical Sciences
Research Group:Analytical chemistry
Research Field:Separation science
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the chemical sciences
UTAS Author:Macka, M (Professor Mirek Macka)
ID Code:152551
Year Published:2021
Web of Science® Times Cited:8
Deposited By:Chemistry
Deposited On:2022-08-22
Last Modified:2022-08-29

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