Yu, RB and Quirino, JP, Open-tubular admicellar electrochromatography of charged analytes, Talanta, 208 Article 120401. ISSN 0039-9140 (2020) [Refereed Article]
© 2019 Published by Elsevier B.V.
Fundamental studies on the separation of cationic and anionic analytes in open-tubular admicellar electrochromatography (OT-AMEC) using cetyltrimethylammonium bromide (CTAB) and fused silica capillaries are presented. OT-AMEC was compared with open-tubular admicellar liquid chromatography (OT-AMLC) by running the two methods using the same mobile phases. The mobile phases were buffered at pH ≥ 6 and contained a low concentration (above the critical surface aggregation concentration and below the critical micelle concentration) of CTAB. The stationary pseudophase of CTAB admicelles were formed at the solid surface and liquid-interface inside the capillary by simply conditioning the capillary with the mobile phase. Separations were performed in a 30 cm (21.5 cm to UV detector) long and 50 μm inner diameter capillary, using low pressure (50 mbar) in OT-AMLC and high voltage (15 kV at negative polarity) in OT-AMEC. The appropriate equations for the experimental estimation of retention factor (k) values of analytes were discussed. For anionic analytes, k in OT-AMEC were carefully determined by considering the observed interaction between CTAB monomers and tested analytes. The calculated k for each analyte was found similar in OT-AMLC and OT-AMEC, although the mechanism of retention was not entirely different due to the contribution of electrophoresis in OT-AMEC. Studies on the addition of a typical (i.e., acetonitrile) and atypical modifier (i.e., nonyl-β-glucoside) into the mobile phase, and sample focusing with >10x improvement in peak height under isocratic conditions were also conducted.
|Item Type:||Refereed Article|
|Keywords:||admicelles, capillary electrochromatography, charged analytes, liquid chromatography, open-tubular, sample focusing|
|Research Division:||Chemical Sciences|
|Research Group:||Analytical chemistry|
|Research Field:||Separation science|
|Objective Division:||Expanding Knowledge|
|Objective Group:||Expanding knowledge|
|Objective Field:||Expanding knowledge in the chemical sciences|
|UTAS Author:||Yu, RB (Mr Raymond Fernando Yu)|
|UTAS Author:||Quirino, JP (Associate Professor Lito Quirino)|
|Web of Science® Times Cited:||8|
|Deposited By:||Research Performance and Analysis|
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