File(s) under permanent embargo
Volatile and non-volatile metabolite changes in 140-day stored vacuum packaged chilled beef and potential shelf life markers
journal contribution
posted on 2023-05-21, 12:16 authored by Frank, D, Hughes, J, Piyasiri, U, Zhang, Y, Mandeep KaurMandeep Kaur, Li, Y, Mellor, G, Stark, JDuring storage of vacuum packaged chilled beef (VPCB), lactic acid bacteria become the dominant microflora, facilitating an extended shelf life. However, at some point, (bio)chemical and organoleptic changes render the meat unacceptable. In this investigation we evaluated volatile and non-volatile metabolite changes in VPCB after 84-, 98-, 120- and 140-days storage at ~ -1 Celsius degree. After 140-days storage, the sensory, volatile and non-volatile data did not indicate spoilage. Minimal changes in volatile signatures of collected weep and on raw and grilled steaks were measured. Changes in selected non-volatile components indicated increased proteolysis (free amino acids, carnosine) and changes in organic acids (lactic, succinic) and nucleotide metabolism. Rapid volatile profiling using proton transfer reaction mass spectrometry showed a clear progression of changes in selected compounds over the storage period. An increased concentration of ethanol and other compounds between 120 and 140 days, suggested that volatile changes may be a useful objective indicator of extended storage VPCB quality.
History
Publication title
Meat ScienceVolume
161Article number
108016Number
108016Pagination
1-11ISSN
0309-1740Department/School
Tasmanian Institute of Agriculture (TIA)Publisher
Elsevier Sci LtdPlace of publication
The Boulevard, Langford Lane, Kidlington, Oxford, England, Oxon, Ox5 1GbRights statement
Crown Copyright 2019 Published by Elsevier LtdRepository Status
- Restricted