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Liquid chromatography with micelles in open-tube capillaries


Quirino, JP and Tarongoy, FM, Liquid chromatography with micelles in open-tube capillaries, Green Chemistry, 20, (11) pp. 2486-2493. ISSN 1463-9262 (2018) [Refereed Article]

Copyright Statement

The Royal Society of Chemistry 2018

DOI: doi:10.1039/c8gc00409a


The greening efforts in analytical chromatography are mostly on the reduction of chemical waste and the replacement of organic solvents used in the mobile phase. Here, we present a new green liquid chromatography approach in open-tubes with the following advantages and/or features: up to zero chemical waste, minimal use of reagents and samples, no preparation of a solid stationary phase, relatively fast separations and reproducible results. The approach was based on the molecular organisation of common long chain ionic surfactants (i.e., hexadecyltrimethylammonium bromide and sodium dodecyl sulfate) to form micelles in solution and at the solid surface-liquid interface. The differential solubilisation or distribution of solutes into these micelles produced the unexpected separation or retention of various types of molecules including aromatic hydrocarbons, anionic, cationic and amphiphilic drugs, pesticides, peptides and a small protein. Chromatography was done in 25-200 mu m range inner diameter and 60 or 120 cm long fused silica capillaries against a flow of predominantly aqueous surfactant solutions above the critical micelle concentration as mobile phase. Distribution between these micelles was affected by the mobile phase conditions such as surfactant concentration, pH, salt concentration and organic solvent content used, which we harnessed to demonstrate sample enrichment. Analytical performance was assessed in the separation of pesticides and antioxidants and in real sample matrices. A comparison with reversed phase chromatography for the analysis of a drug combination was also done. This important fundamental behavior of surfactant micelles presented might find use in the development of green extraction technologies, nano-micro separations and portable devices.

Item Details

Item Type:Refereed Article
Keywords:electrokinetic chromatography, chemical relaxation, stationary phases, mobile phases, waste-water, columns, separation, surfactants, solvents, electrochromatography
Research Division:Chemical Sciences
Research Group:Analytical chemistry
Research Field:Separation science
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the chemical sciences
UTAS Author:Quirino, JP (Associate Professor Lito Quirino)
ID Code:152335
Year Published:2018
Web of Science® Times Cited:22
Deposited By:Chemistry
Deposited On:2022-08-17
Last Modified:2022-09-20

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