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Physico-chemical properties of late-incubation egg amniotic fluid and a potential in ovo feed supplement


Omede, AA and Bhuiyan, MM and Islam, AF and Iji, PA, Physico-chemical properties of late-incubation egg amniotic fluid and a potential in ovo feed supplement, Asian - Australasian Journal of Animal Sciences, 30, (8) pp. 1124-1134. ISSN 1011-2367 (2017) [Refereed Article]

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This study explored the physico-chemical properties of late-incubation egg amniotic fluid and a potential in ovo feed (IOF) supplement.


Amniotic fluid was collected from broiler breeders (Ross 308, 51 weeks and Cobb 500, 35 weeks) on day 17 after incubation. A mixture of high-quality soy protein supplement Hamlet Protein AviStart (HPA) was serially diluted in MilliQ water to obtain solutions ranging from 150 to 9.375 mg/mL. The mixtures were heat-treated (0, 30, 60 minutes) in a waterbath (80C) and then centrifuged to obtain supernatants. The amniotic fluid and HPA supernatants were analysed for their physico-chemical properties.


Only viscosity and K+ were significantly (p<0.05) different in both strains. Of all essential amino acids, leucine and lysine were in the highest concentration in both strains. The osmolality, viscosity and pCO2 of the supernatants decreased (p<0.05) with decreasing HPA concentration. Heat treatment significantly (p<0.05) affected osmolality, pH, and pCO2, of the supernatants. The interactions between HPA concentration and heat treatment were significant with regards to osmolality (p<0.01), pH (p<0.01), pCO2 (p<0.05), glucose (p<0.05), lactate (p<0.01) and acid-base status (p<0.01) of HPA solutions. The Ca2+, K+, glucose, and lactate increased with increasing concentration of HPA solution. The protein content of HPA solutions decreased (p<0.05) with reduced HPA solution concentrations. The supernatant from 150 mg/mL HPA solution was richest in glutamic acid, aspartic acid, arginine and lysine. Amino acids concentrations were reduced (p<0.05) with each serial dilution but increased with longer heating.


The values obtained in the primary solution (highest concentration) are close to the profiles of high-protein ingredients. This supplement, as a solution, hence, may be suitable for use as an IOF supplement and should be tested for this potential.

Item Details

Item Type:Refereed Article
Keywords:amniotic fluid, broiler, in ovo, physico-chemical properties
Research Division:Agricultural, Veterinary and Food Sciences
Research Group:Animal production
Research Field:Animal nutrition
Objective Division:Animal Production and Animal Primary Products
Objective Group:Livestock raising
Objective Field:Poultry
UTAS Author:Omede, AA (Dr Apeh Omede)
ID Code:152201
Year Published:2017
Deposited By:Agriculture and Food Systems
Deposited On:2022-08-12
Last Modified:2022-09-05

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