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Cryopreservation moderates the thrombogenicity of arterial allografts during storage

Citation

Hidi, L and Komorowicz, E and Kovacs, GI and Szeberin, Z and Garbaisz, D and Nikolova, N and Tenekedjiev, K and Szabo, L and Kolev, K and Sotonyi, P, Cryopreservation moderates the thrombogenicity of arterial allografts during storage, PLoS ONE, 16, (7) Article e0255114. ISSN 1932-6203 (2021) [Refereed Article]


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Copyright Statement

2021 Hidi et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International (CC BY 4.0) License, (https://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

DOI: doi:10.1371/journal.pone.0255114

Abstract

Introduction:

Management of vascular infections represents a major challenge in vascular surgery. The use of cryopreserved vascular allografts could be a feasible therapeutic option, but the optimal conditions for their production and use are not precisely defined. Aims

To evaluate the effects of cryopreservation and the duration of storage on the thrombogenicity of femoral artery allografts.

Methods

In our prospective study, eleven multi-organ-donation-harvested human femoral arteries were examined at five time points during storage at -80C: before cryopreservation as a fresh native sample and immediately, one, twelve and twenty-four weeks after the cryopreservation. Cross-sections of allografts were perfused with heparin-anticoagulated blood at shear-rates relevant to medium-sized arteries. The deposited platelets and fibrin were immunostained. The thrombogenicity of the intima, media and adventitia layers of the artery grafts was assessed quantitatively from the relative area covered by fibrin- and platelet-related fluorescent signal in the confocal micrographs.

Results

Regression analysis of the fibrin and platelet coverage in the course of the 24-week storage excluded the possibility for increase in the graft thrombogenicity in the course of time and supported the hypothesis for a descending trend in fibrin generation and platelet deposition on the arterial wall. The fibrin deposition in the cryopreserved samples did not exceed the level detected in any of the three layers of the native graft. However, an early (up to week 12) shift above the native sample level was observed in the platelet adhesion to the media.

Conclusions

The hemostatic potential of cryopreserved arterial allografts was retained, whereas their thrombogenic potential declined during the 6-month storage. The only transient prothrombotic change was observed in the media layer, where the platelet deposition exceeded that of the fresh native grafts in the initial twelve weeks after cryopreservation, suggesting a potential clinical benefit from antiplatelet therapy in this time-window.

Item Details

Item Type:Refereed Article
Research Division:Biomedical and Clinical Sciences
Research Group:Other biomedical and clinical sciences
Research Field:Other biomedical and clinical sciences not elsewhere classified
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the biomedical and clinical sciences
UTAS Author:Nikolova, N (Professor Nataliya Nikolova)
UTAS Author:Tenekedjiev, K (Professor Kiril Tenekedjiev)
ID Code:152005
Year Published:2021
Web of Science® Times Cited:1
Deposited By:Plant Science
Deposited On:2022-08-09
Last Modified:2022-09-02
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