Hidi, L and Komorowicz, E and Kovacs, GI and Szeberin, Z and Garbaisz, D and Nikolova, N and Tenekedjiev, K and Szabo, L and Kolev, K and Sotonyi, P, Cryopreservation moderates the thrombogenicity of arterial allografts during storage, PLoS ONE, 16, (7) Article e0255114. ISSN 1932-6203 (2021) [Refereed Article]
© 2021 Hidi et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International (CC BY 4.0) License, (https://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Introduction:Management of vascular infections represents a major challenge in vascular surgery. The use of cryopreserved vascular allografts could be a feasible therapeutic option, but the optimal conditions for their production and use are not precisely defined. Aims To evaluate the effects of cryopreservation and the duration of storage on the thrombogenicity of femoral artery allografts.
MethodsIn our prospective study, eleven multi-organ-donation-harvested human femoral arteries were examined at five time points during storage at -80C: before cryopreservation as a fresh native sample and immediately, one, twelve and twenty-four weeks after the cryopreservation. Cross-sections of allografts were perfused with heparin-anticoagulated blood at shear-rates relevant to medium-sized arteries. The deposited platelets and fibrin were immunostained. The thrombogenicity of the intima, media and adventitia layers of the artery grafts was assessed quantitatively from the relative area covered by fibrin- and platelet-related fluorescent signal in the confocal micrographs.
ResultsRegression analysis of the fibrin and platelet coverage in the course of the 24-week storage excluded the possibility for increase in the graft thrombogenicity in the course of time and supported the hypothesis for a descending trend in fibrin generation and platelet deposition on the arterial wall. The fibrin deposition in the cryopreserved samples did not exceed the level detected in any of the three layers of the native graft. However, an early (up to week 12) shift above the native sample level was observed in the platelet adhesion to the media.
ConclusionsThe hemostatic potential of cryopreserved arterial allografts was retained, whereas their thrombogenic potential declined during the 6-month storage. The only transient prothrombotic change was observed in the media layer, where the platelet deposition exceeded that of the fresh native grafts in the initial twelve weeks after cryopreservation, suggesting a potential clinical benefit from antiplatelet therapy in this time-window.
|Item Type:||Refereed Article|
|Research Division:||Biomedical and Clinical Sciences|
|Research Group:||Other biomedical and clinical sciences|
|Research Field:||Other biomedical and clinical sciences not elsewhere classified|
|Objective Division:||Expanding Knowledge|
|Objective Group:||Expanding knowledge|
|Objective Field:||Expanding knowledge in the biomedical and clinical sciences|
|UTAS Author:||Nikolova, N (Professor Nataliya Nikolova)|
|UTAS Author:||Tenekedjiev, K (Professor Kiril Tenekedjiev)|
|Web of Science® Times Cited:||1|
|Deposited By:||Plant Science|
|Downloads:||1 View Download Statistics|
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