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Pressurized hot water extraction and capillary electrophoresis for green and fast analysis of useful metabolites in plants


Debruille, K and Smith, Jason Alfred and Quirino, JP, Pressurized hot water extraction and capillary electrophoresis for green and fast analysis of useful metabolites in plants, Molecules, 24, (13) pp. 1-12. ISSN 1420-3049 (2019) [Refereed Article]

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Copyright Statement

Copyright: 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons 4.0 International (CC BY 4.0) license (

DOI: doi:10.3390/molecules24132349


The search for useful compounds from plants is an important research area. Traditional screening that involves isolation and identification/quantitation is tedious, time consuming, and generates a significant amount of chemical waste. Here, we present a simple, fast, and green strategy to assess >= 0.1% wt/wt quantities of useful compounds in plants/spices using pressurized hot water extraction using a household espresso machine followed by chemical analysis using capillary electrophoresis. Three demonstrations with polygodial, cinnamaldehyde, coumarin, and shikimic acid as target metabolites are shown. Direct analysis of extracts was by the developed micellar electrokinetic chromatography and capillary zone electrophoresis methods. The approach, which can be implemented in less developed countries, can process many samples within a day, much faster than traditional techniques that would normally take at least a day. Finally, 0.8-1.1% wt/wt levels of shikimic acid were found in Tasmanian-pepperberry and Tasmanian-fuschia leaves via the approach.

Item Details

Item Type:Refereed Article
Keywords:plant metabolites, pressurized hot water extraction, capillary electrophoresis, polygodial, coumarin, shikimic acid
Research Division:Chemical Sciences
Research Group:Medicinal and biomolecular chemistry
Research Field:Biologically active molecules
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the chemical sciences
UTAS Author:Smith, Jason Alfred (Associate Professor Jason Smith)
UTAS Author:Quirino, JP (Associate Professor Lito Quirino)
ID Code:151975
Year Published:2019
Web of Science® Times Cited:3
Deposited By:Chemistry
Deposited On:2022-08-09
Last Modified:2022-09-29

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